TABLE 3 Developmental Capacity of Cytoplasts Reconstituted with Nuclei from Embryonic Cells

1

2

3

4

5

Species

Recipient cytoplast

Donor cell type

% Early Development: % Blastocyst (# Blastocysts/ # Cultured)

% Term Development: % Offspring (# Live births/ # Transferred)

Mouse

Zygote

Inner cell mass

16% (23/142)

19% (3/16)

Trophectoderm

1% (1/68)

0

Zygote

Pronuclear

95% (20/21)

no transfer

2-cell

13% (19/151)

no transfer

4-cell

0 (0/81)

no transfer

8-cell

0 (0/111)

no transfer

Inner cell mass

0 (0/84)

no transfer

Zygote

8-cell

0(0/32)

no transfer

Inner cell mass

0 (0/84)

no transfer

Zygote

8-cell

0(0/32)

no transfer

2-cell blastomere

2-cell

93% (40/43)

24%a (10/41)

Zygote

8-cell

51% (45/89)

0a (0/11)

Cumulus cell

0 (0/91)

no transfer

2-cell blastomere

4-cell

72% (49/68)

22% (10/46)

8-cell

35% (49/139)

8% (4/48)

Inner cell mass

0 (0/91)

no transfer

Mll oocyte

2-cell

23% (20/88)

15% (3/20)

8-cell

4% (1/26)

0 (0/1)

Inner cell mass

13% (11/87)

0 (0/11)

2-cell

78% (36/46)

29% (10/34)

4-cell

71% (30/42)

22% (6/27)

8-cell

46% (18/39)

18% (3/17)

4-cellc

83% (58/70)

43% (2/58)

Inner cell massc

64% (23/36)

11% (2/18)

Trophectodermc

62% (16/26)

8% (2/25)

ES cell

5% (47/931)

0 (0/56)

ES cell

29% (312/1087)

6% (8/132)

Sheep

Mll oocyte

8-cell

33% (8/24)

75% (3/4)

 

16-cell

27% (13/49)

21% (3/14)



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