imaging studies, 83, 84

inhalation anesthetics, 42, 104, 105, 106, 108

injectable anesthetics, 42, 106

lesions, 98

local anesthesia, 22, 43, 96, 98

multiple major survival surgeries, 32

neurophysiologic recording, 75

professional training, 30, 31

perinatal studies, 102, 103, 104-106, 108

prolonged nonsurvival studies, 88-91 (passim)

protocols, 17, 20, 22

regulatory oversight, 11, 17, 43-44

surgery, 11, 17, 32, 37, 41, 42-44, 45, 46, 75, 104

U.S. Government Principles, 42

vocalizations, 106

Animal Care Policies (APHIS/AC Policies), 7-8, 10-11, 17-18

food regulation, 52

prolonged nonsurvival studies, 92

surgeries, 41, 92

Animal husbandry, see Husbandry and nursing care

Animal and Plant health Inspection Service, 7

see also Animal Care Policies (APHIS/ AC Policies), 7-8, 10-11, 17-18

Animal Welfare Act, enforcement, 7

Animal Care Panel, 8

Animal Welfare Act (AWA), viii, 3, 7-11 (passim), 17-18, 39, 41

Animal Welfare Regulations (AWRs)

anesthetics and analgesics, 43-44

euthanasia, 26-27

fetal surgery, 107-108

food regulation, 52

oversight agencies, general, 7, 8, 9, 12

pain and distress, 17

physical restraint, 49

professional training, 30

prolonged nonsurvival studies, 92

protocols, 13, 17, 39-40

social disruption, 145

surgery, 37-45 (passim), 107-108

Anxiety, 21, 23

behavioral screening of genetically modified animals, 135-136

housing of multiple species, 36-37

physical restraint, 48

Anxiolytics, 23, 43, 90, 135

Appetitive stimuli, 123-125, 130, 138

animal care and use concerns, 126-128

protocols, 126

reasons for, 125-126

terminology, 123-125

Aseptic techniques, 32, 37, 38, 44-45

implants and probes, 73, 76, 80-82, 92-93, 97

lesions, 87

neurophysiologic recording in awake, behaving animals, 72, 73, 75, 78, 79-80, 81-82

professional training, 31

prolonged nonsurvival studies, 76, 89, 92-93

surgery, 37, 38, 40, 44-45, 72, 73, 75, 78, 79

Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC), 9-10

Automated systems, see Computer applications and automated systems

Aversive stimuli, 138-139

animal care and use concerns, 52, 126-128

food and fluid regulation, 52

neural injury and diseases, 100, 101

protocol development, 21, 28

reasons for, 125-126

sound, 117

terminology, 123-125

Awake, behaving animals

see also Neurophysiologic recording in awake, behaving animals;

Physical restraints

animal care and use concerns, 76

occupational health and safety concerns, 82

zoonoses, protocols, 29, 46, 85

B

Behavior, vii, viii, 1

see also Awake behaving animals;

Distress;

terms beginning “Behavior...”

addictive drugs, 115, 142

disease models, 94-95, 96

distress assessment, protocols, 21, 22

fluid regulation, 50, 51, 57

Front Matter (R1-R14)

Introduction (1-4)

1 Regulatory and Ethical Considerations (5-11)

2 Protocol-Development Strategies (12-29)

3 General Animal-Care Concerns (30-68)

4 Survival Studies (69-87)

5 Prolonged Nonsurvival Studies (88-93)

6 Studies of Neural Injury and Disease (94-101)

7 Perinatal Studies (102-108)

8 Agents and Treatments (109-122)

9 Behavioral Studies (123-149)

References (150-174)

Appendix A: Sample Size Determination (175-180)

Appendix B: Estimating Animal Numbers (181-190)

Appendix C: Biographical Sketches of Authoring Committee (191-194)

Index (195-209)