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Health Risks from Exposure to Low Levels of Ionizing Radiation: Beir VII Phase 2 Appendixes
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Health Risks from Exposure to Low Levels of Ionizing Radiation: Beir VII Phase 2 A Basic Biological and Genetic Concepts DNA, Genes, and Chromosomes The genetic material of living organisms, DNA, is contained in chromosomes, which are present in the nuclei of cells. Chromosomes contain genes, which are the basic units of inheritance. Humans have 23 pairs of chromosomes: one member of each pair derived from the father and the other from the mother. Males have 22 pairs of autosomes and an X and a Y chromosome (the latter two are called sex chromosomes). Females have 22 pairs of autosomes and two X chromosomes. Ordinary body cells (somatic cells) contain the full complement of 23 pairs of chromosomes (referred to as the diploid number), whereas the mature germ cells—sperm and ova—contain only half the diploid number of chromosomes (referred to as the haploid number) that consists of 3 × 109 base pairs (bp) of DNA. Each of the genes occupies a specific position in a specific chromosome called the locus (plural loci). The two genes at each locus, one paternal and one maternal, are called alleles. The totality of all the genes is the genotype of the individual, and their manifestation is the phenotype. Most eukaryotic (including human) genes are made up of sequences (exons) that code for amino acid sequences in proteins and noncoding intervening sequences (introns). Genes differ not only in the DNA sequences that specify the amino acids of the proteins they encode but also in their structures. A few human genes, such as histone genes, interferon genes, and mitochondrial genes, do not contain introns; some contain a considerable number of introns whose lengths vary from a few bases to several kilobases (kb; e.g., the dystrophin gene, DMD, mutations in which result in Duchenne’s and Becker’s muscular dystrophies, is 2400 kb long and contains 79 introns). The 5′ end of the gene is marked by the translational start site (the ATG codon). Upstream from this are a number of noncoding sequences referred to as promoters; further upstream are a number of cis-acting regulatory elements of defined sequence (TATAAA and CCAAAT motifs), which play a role in constitutive gene expression, and enhancers, which respond to particular proteins in a tissue-specific manner by increasing transcription. At the 3′ end is the termination codon (e.g., TAA, TAG, TGA) and a poly-A tail. The process by which genetic information in DNA is used to produce amino acids and proteins is called transcription. During this process, the entire unit of both introns and exons is transcribed into precursor messenger RNA (mRNA). The region of the precursor mRNA transcribed from the introns is then excised and removed and does not form the definitive mRNA. Precursor mRNA from the exons is spliced together to form the definitive mRNA, which specifies the primary structure of the gene product. The definitive mRNA is then transported to the cytoplasm, where protein synthesis occurs. Mutations and Their Effects on the Phenotype Mutations are permanent heritable changes that occur in the genetic material. They arise spontaneously and can be induced by exposure to radiation or chemical mutagens. When mutations arise or are induced in somatic cells, there is a very small probability that they will cause cancer, but somatic mutations are not transmitted to progeny. If mutations occur or are induced in germ cells, they can be transmitted to progeny and they may result in genetic (hereditary) diseases. Mutations are classified as dominant or recessive, depending on their effects on the phenotype (physical appearance of the organism). In the case of a dominant mutation, a single mutant allele inherited from either parent is sufficient to cause an altered phenotype; the organism has one mutant and one normal allele of the gene in question and is called a heterozygote with respect to that gene. In the case of a recessive mutation, two mutant alleles of the same gene—one from each parent—are required to produce a mutant phenotype; the organism is called a homozygote for the gene. In general, mutations in genes that code for structural proteins are dominant, and those in genes that code for enzymatic proteins are recessive.
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Health Risks from Exposure to Low Levels of Ionizing Radiation: Beir VII Phase 2 Genetic Diseases Genetic diseases are traditionally classified as Mendelian or multifactorial diseases. Mendelian diseases are due to mutations in single genes; multifactorial diseases arise as a result of the joint action of multiple genetic and environmental factors. Molecular analyses have revealed that a wide variety of mutational changes underlie Mendelian diseases: “microlesions,” such as single base-pair substitutions, deletions, insertions, or duplications involving one to a few base pairs; and “gross lesions,” such as whole-gene or multigene deletions, complex rearrangements, and large insertions and duplications. Microlesions dominate the spectrum of Mendelian diseases (Krawczak and Cooper 1997). At the functional level, mutations can be classified as causing either a loss of function or the gain of a new function. Normal gene function can be abolished by some types of point mutations, partial or total gene deletions, disruption of the gene structure by translocations or inversions of the genetic material, and so on. In most cases, loss-of-function mutations in enzyme-coding genes are recessive, because 50% of the gene product is usually sufficient for normal functioning. Loss-of-function mutations in genes that code for structural or regulatory proteins, however, result in dominant phenotypes through haploinsufficiency (a 50% reduction in the gene product in the heterozygote is insufficient for normal functioning but is compatible with viability) or through dominant negative effects (the product of the mutant gene not only loses its own function but also prevents the product of the normal allele from functioning in a heterozygous organism). Dominant negative effects are seen particularly in the case of genes whose products function as aggregates (dimers and multimers). In contrast, gain of function is likely when only specific changes cause a given disease phenotype. Gains of truly novel functions are not common except in cancer, but in inherited diseases, gain of function usually means that the mutant gene is expressed at the wrong time in development, in the wrong tissue, in response to wrong signals, or at an inappropriately high level. The spectrum of gain-of-function mutations would therefore be more restricted, and deletion or disruption of the gene would not produce the disease. Genetic Effects of Radiation Exposure of cells and organisms to ionizing radiation causes DNA damage. The cellular processing of radiation-induced damage to DNA by enzymes may result in a return to normal sequence and structure (Lobrich and others 1995), or processing may fail or may cause alterations in DNA that lead to lethality or heritable changes (mutations and chromosomal aberrations) in surviving cells. Heritable changes induced in reproductive (germ) cells can be transmitted to the following generations and cause genetic disease of one kind or another (a concept that lies at the core of estimation of the genetic risks posed by radiation). Changes induced in nonreproductive (somatic) cells have a small but finite probability of contributing to the complex process of carcinogenesis. The types of mutational changes induced by radiation are broadly similar to the types that occur naturally, but the proportions of the different types are not the same. The results of molecular studies of radiation-induced germ cell mutations in experimental organisms and in mammalian somatic cells support the view that most radiation-induced mutations involve changes in large segments of the DNA, such as deletions that often encompass more than one gene. Hence, radiation readily induces the kinds of molecular changes that can derange a genome and lead to cancer. Conversely, many of those changes, if they occur in germ cells, are incompatible with embryo development and result in developmental abnormalities or lethal mutations in the germline, which would result in nonviable progeny.
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