APPLICATION OF TOXICOGENOMICS TO CROSS-SPECIES EXTRAPOLATION
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NOTICE: The project that is the subject of this report was approved by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, and the Institute of Medicine. The members of the committee responsible for the report were chosen for their special competences and with regard for appropriate balance.
This project was supported by Contract No. N01-OD-4-2139 between the National Academy of Sciences and National Institute of Environmental Health Sciences. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the organizations or agencies that provided support for this project.
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COMMITTEE ON APPLICATIONS OF TOXICOGENOMICS TO CROSS-SPECIES EXTRAPOLATION: A WORKSHOP
Members
N. LEIGH ANDERSON (Chair),
Plasma Proteome Institute, Washington, DC
JAMES S. BUS,
Dow Chemical Company, Midland, MI
DAVID L. EATON,
University of Washington, Seattle
SERRINE S. LAU,
University of Arizona, Tucson
JOHN A. MOORE,
Hollyhouse, Inc., Wicomico Church, VA
JOHN QUACKENBUSH,
Harvard School of Public Health, Boston, MA
KENNETH S. RAMOS,
University of Louisville, Louisville, KY
Staff
MARILEE SHELTON-DAVENPORT, Project Director
ROBERTA WEDGE, Project Director
NORMAN GROSSBLATT, Senior Editor
JENNIFER E. SAUNDERS, Research Associate
MIRSADA KARALIC-LONCAREVIC, Research Associate
ALEXANDRA STUPPLE, Senior Editorial Assistant
LUCY V. FUSCO, Senior Program Assistant
JORDAN CRAGO, Senior Program Assistant
SAMMY BARDLEY, Librarian
Sponsor
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
COMMITTEE ON EMERGING ISSUES AND DATA ON ENVIRONMENTAL CONTAMINANTS
Members
KENNETH S. RAMOS (Chair),
University of Louisville, Louisville, KY
N. LEIGH ANDERSON,
Plasma Proteome Institute, Washington, DC
PATRICIA A. BUFFLER,
University of California, Berkeley
JAMES S. BUS,
Dow Chemical Company, Midland, MI
JOSEPH DEGEORGE,
Merck Pharmaceuticals, West Point, PA
GEORGIA M. DUNSTON,
Howard University, Washington, DC
LINDA E. GREER,
Natural Resources Defense Council, Washington, DC
ROBERT J. GRIFFIN,
Marquette University, Milwaukee, WI
JOHN D. GROOPMAN,
Johns Hopkins University, Baltimore, MD
CASIMIR A. KULIKOWSKI,
Rutgers University, New Brunswick, NJ
AMY D. KYLE,
University of California, Berkeley
PHILIP LEDER,
Harvard Medical School, Boston, MA
PETER LORD,
Johnson & Johnson, Raritan, NJ
WILLIAM B. MATTES,
Gene Logic, LLC, Gaithersburg, MD
GEORGE ORPHANIDES,
Syngenta, LLC, Cheshire, UK
FREDERICA P. PERERA,
Columbia University, New York, NY
JOHN QUACKENBUSH,
Harvard School of Public Health, Boston, MA
MARK A. ROTHSTEIN,
University of Louisville School of Medicine, Louisville, KY
LEONA D. SAMSON,
Massachusetts Institute of Technology, Cambridge
PETER S. SPENCER,
Oregon Health Sciences Institute, Portland
LAWRENCE SUNG,
University of Maryland, Baltimore
CHERYL L. WALKER,
University of Texas, Smithville
RUSSELL D. WOLFINGER,
SAS Institute, Inc., Cary, NC
Staff
MARILEE SHELTON-DAVENPORT, Project Director
KARL GUSTAVSON, Project Director
EILEEN ABT, Program Director
NORMAN GROSSBLATT, Senior Editor
JENNIFER E. SAUNDERS, Research Associate
LUCY V. FUSCO, Senior Program Assistant
JORDAN CRAGO, Senior Program Assistant
BOARD ON ENVIRONMENTAL STUDIES AND TOXICOLOGY
Members
JONATHAN M. SAMET (Chair),
The Johns Hopkins University, Baltimore, MD
RAMÓN ALVAREZ,
Environmental Defense, Austin, TX
THOMAS BURKE,
The Johns Hopkins University, Baltimore, MD
JUDITH C. CHOW,
Desert Research Institute, Reno, NV
COSTEL D. DENSON,
University of Delaware, Newark
E. DONALD ELLIOTT,
Willkie Farr & Gallagher LLP, Washington, DC
CHRISTOPHER B. FIELD,
Carnegie Institution of Washington, Stanford, CA
SHERRI W. GOODMAN,
Center for Naval Analyses, Alexandria, VA
JUDITH A. GRAHAM,
American Chemistry Council, Arlington, VA
DANIEL S. GREENBAUM,
Health Effects Institute, Cambridge, MA
ROBERT HUGGETT,
Michigan State University, East Lansing
BARRY L. JOHNSON,
Emory University, Atlanta, GA
JAMES H. JOHNSON,
Howard University, Washington, DC
JUDITH L. MEYER,
University of Georgia, Athens
PATRICK Y. O’BRIEN,
ChevronTexaco Energy Technology Company, Richmond, CA
DOROTHY E. PATTON,
International Life Sciences Institute, Washington, DC
STEWARD T.A. PICKETT,
Institute of Ecosystem Studies, Millbrook, NY
JOSEPH V. RODRICKS,
ENVIRON Corporation, Arlington, VA
ARMISTEAD G. RUSSELL,
Georgia Institute of Technology, Atlanta
LISA SPEER,
Natural Resources Defense Council, New York, NY
KIMBERLY M. THOMPSON,
Harvard School of Public Health, Boston, MA
G. DAVID TILMAN,
University of Minnesota, St. Paul
CHRIS G. WHIPPLE,
ENVIRON Corporation, Emeryville, CA
LAUREN A. ZEISE,
California Environmental Protection Agency, Oakland
Senior Staff
JAMES J. REISA, Director
DAVID J. POLICANSKY, Scholar
RAYMOND A. WASSEL, Senior Program Officer for Environmental Sciences and Engineering
KULBIR BAKSHI, Senior Program Officer for Toxicology
EILEEN N. ABT, Senior Program Officer for Risk Analysis
K. JOHN HOLMES, Senior Program Officer
SUSAN N.J. MARTEL, Senior Program Officer
SUZANNE VAN DRUNICK, Senior Program Officer
ELLEN K. MANTUS, Senior Program Officer
RUTH E. CROSSGROVE, Senior Editor
BOARD ON LIFE SCIENCES
Members
CORY S. GOODMAN (Chair),
Renovis Inc., South San Francisco, CA
ANN M. ARVIN,
Stanford University School of Medicine, Stanford, CA
JEFFREY L. BENNETZEN,
University of Georgia, Athens, GA
RUTH BERKELMAN,
Emory University, Atlanta, GA
R. ALTA CHARO,
University of Wisconsin, Madison
DENNIS CHOI,
Merck Research Laboratories, West Point, PA
JEFFREY L. DANGL,
University of North Carolina, Chapel Hill
PAUL R. EHRLICH,
Stanford University, Palo Alto, CA
JAMES M. GENTILE,
Hope College, Holland, MI
ED HARLOW,
Harvard Medical School, Boston, MA
DAVID HILLIS,
University of Texas, Austin
KENNETH F. KELLER,
University of Minnesota, Minneapolis
RANDALL MURCH,
Institute for Defense Analyses, Alexandria, VA
GREGORY A. PETSKO,
Brandeis University, Waltham, MA
STUART L. PIMM,
Duke University, Durham, NC
BARBARA A. SCHAAL,
Washington University, St. Louis, MO
JAMES TIEDJE,
Michigan State University, East Lansing
KEITH YAMAMOTO,
University of California, San Francisco
Senior Staff
FRANCES E. SHARPLES, Director
KERRY A. BRENNER, Senior Program Officer
ROBIN A. SCHOEN, Senior Program Officer
MARILEE SHELTON-DAVENPORT, Senior Program Officer
ROBERT T. YUAN, Senior Program Officer
ADAM P. FAGEN, Program Officer
ANN REID, Program Officer
EVONNE P.Y. TANG, Program Officer
SETH STRONGIN, Senior Program Assistant
DENISE GROSSHANS, Financial Associate
Preface
Toxicogenomics has been described as a discipline combining expertise in toxicology, genetics, molecular biology, and environmental health to elucidate the response of living organisms to stressful environments. It includes, but is not limited to, the study of how genomes respond to toxicant exposures and how genotype affects responses to toxicant exposures. As the technology rapidly develops, it is critical that scientists and the public communicate about the promises and limitations of this new field. Despite the dependence on animal models in toxicologic research for predicting human health effects in the regulatory arena, there can be important differences between how animals and humans respond to different chemicals. The Committee on Applications of Toxicogenomics to Cross-Species Extrapolation designed a workshop to consider using toxicogenomics in cross-species extrapolation from animals to humans. The workshop reflected on the promises and limitations of emerging data-rich approaches—such as genotyping (genomics), mRNA analysis (transcriptomics), protein analysis (proteomics), and metabolite analysis (metabolomics)—to inform cross-species extrapolation. Specifically, the workshop considered whether the data-rich technologies offer new ways of determining whether the effects of chemicals in test animals can be used to predict human responses.
This workshop report has been reviewed in draft form by persons chosen for their diverse perspectives and technical expertise in accordance with procedures approved by the National Research Council’s Report Review Committee. The purpose of this independent review is to provide candid and critical comments that will assist the institution in making its published workshop report as sound as possible and to ensure
that the report meets institutional standards of objectivity, evidence, and responsiveness to the study charge. The review comments and draft manuscript remain confidential to protect the integrity of the deliberative process. We wish to thank the following people for their review of this workshop report: Susan Sumner, RTI International; Jonathan H. Freedman, Duke University; Kevin W. Gaido, CIIT Centers for Integrated Genomics; and Frank A. Witzmann, Indiana University School of Medicine.
Although the reviewers listed above have provided many constructive comments and suggestions, they were not asked to endorse the conclusions or recommendations, nor did they see the final draft of the workshop report before its release. The review of the workshop report was overseen by Rogene Henderson, of the Lovelace Respiratory Research Institute. Appointed by the National Research Council, she was responsible for making certain that an independent examination of the workshop report was carried out in accordance with institutional procedures and that all review comments were carefully considered. Responsibility for the final content of the workshop report rests entirely with the committee and the institution.
The committee gratefully acknowledges the following for making presentations at the workshop: John L. Butenhoff, 3M Company; Frank A. Witzmann, Indiana University School of Medicine; William H. Benson, Stephen Nesnow, and Kerry L. Dearfield, U.S. Environmental Protection Agency; Richard T. Di Giulio, Duke University; Donna Mendrick, Gene Logic Inc.; Susan Sumner, RTI International; and Russell Thomas, CIIT Centers for Health Research.
The committee is grateful for the assistance of the National Research Council staff in preparing this workshop summary: Marilee Shelton-Davenport, Roberta Wedge, and Karl Gustavson, project directors; James Reisa, director of the Board on Environmental Studies and Toxicology; Fran Sharples, director of the Board on Life Sciences; Jennifer Saunders and Mirsada Karalic-Loncarevic, research associates; Jennifer Roberts, postdoctoral research associate; Norman Grossblatt, senior editor; Lucy Fusco and Jordan Crago, senior program assistants; and Sammy Bardley, librarian.
Finally, I thank the members of the committee for their dedicated efforts throughout the development of this workshop summary.
N. Leigh Anderson
Chair, Committee on Application of Toxicogenomics to Cross-Species Extrapolation