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18
Structured Lipids: An Overview and Comments on Performance Enhancement Potential

Ronald J.Jandacek1

INTRODUCTION

This examination of the role of structured lipids in performance enhancement defines structured lipids and compares their properties and metabolism with those of typical long-chain dietary fats. The potential use of structured lipids in diets intended to enhance performance is examined in this chapter in light of the current understanding of fat metabolism and the relatively small amount of information now available on the specific use of structured lipids in performance enhancement.

The primary focus of research with structured lipids has been in the areas of parenteral and enteral nutritional regimens for the stresses caused by surgery, burns, and trauma. A review of results of these uses of structured lipids is included in this chapter to give a complete picture of the metabolism of structured lipids.

1  

Ronald J.Jandacek, Research Chemist, Procter & Gamble Co., Miami Valley Laboratories, P.O. Box 398707, Cincinnati, OH 45239–8707



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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations 18 Structured Lipids: An Overview and Comments on Performance Enhancement Potential Ronald J.Jandacek1 INTRODUCTION This examination of the role of structured lipids in performance enhancement defines structured lipids and compares their properties and metabolism with those of typical long-chain dietary fats. The potential use of structured lipids in diets intended to enhance performance is examined in this chapter in light of the current understanding of fat metabolism and the relatively small amount of information now available on the specific use of structured lipids in performance enhancement. The primary focus of research with structured lipids has been in the areas of parenteral and enteral nutritional regimens for the stresses caused by surgery, burns, and trauma. A review of results of these uses of structured lipids is included in this chapter to give a complete picture of the metabolism of structured lipids. 1   Ronald J.Jandacek, Research Chemist, Procter & Gamble Co., Miami Valley Laboratories, P.O. Box 398707, Cincinnati, OH 45239–8707

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations Investigations into the use of structured lipids to enhance physical performance in normal subjects have been limited to the replacement of typical fats or carbohydrates by the significant fatty acid component of structured lipids: medium-chain fatty acids. These few studies are summarized, and their results are discussed. This chapter first discusses the metabolism of typical long-chain triacylglycerol (LCT) fats. It then compares the metabolism and properties of medium-chain fatty acids and medium-chain triacylglycerols (MCTs) with those of LCTs. Structured lipids are then defined, and their uses in enteral and parenteral nutrition are reviewed. Finally, the data from the use of MCTs in exercise trials are reviewed and discussed. THE METABOLISM OF TYPICAL LCT FATS Most of the fats in the human diet are triacylglycerol compounds, which are glycerol esters of fatty acids with 12 to 18 carbon atoms. A class of triacylglycerols that occurs occasionally in natural fats and oils has been made synthetically by esterification of glycerol with a mixture of long-chain fatty acids (12–18 carbon atoms) and of medium-chain fatty acids (6–10 carbon atoms). The metabolism of these mixed fatty acid triacylglycerols, known as structured lipids, has been compared with that of typical LCTs (Figure 18–1). FIGURE 18–1 Structure of a typical long-chain triacylglycerol fatty acid.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations To assess the metabolism of structured lipids, it is useful to review the areas of fat metabolism in which structured lipids may differ significantly from normal fats. The utilization of typical LCT fats begins with ingestion and transit to the stomach where a gastric or lingual lipase begins to catalyze the hydrolysis of a small fraction of the fat to diacylglycerol and free fatty acid (Hamosh et al., 1975). This hydrolysis may be more important in the infant than in the adult. Fat is released into the small intestine more slowly than water-miscible substances, and here most of the fat is digested and absorbed. The coarse emulsion of fat is converted into a much finer dispersion of oil droplets through mechanical mixing and the reduction of interfacial tension as bile salts, and phospholipids are introduced through gallbladder contraction. This dispersion of fat greatly increases the interfacial area where pancreatic lipase can act to catalyze the hydrolysis of the triacylglycerol into the 2-monoacylglycerol and fatty acids from the 1 and 3 positions of the glycerol. These digestion products become part of mixed micelles of bile salts and phospholipid—molecular aggregates that are compatible with theaqueous milieu of the intestine but that contain lipophilic components. These mixed micelles are responsible for the transport of fatty acids and 2-monoacylglycerol through the aqueous medium to the intestinal mucosal cell. Micellar transport also facilitates the transport of the fat digestion products through an unstirred water layer that hinders the absorption of hydrophobic compounds (Figure 18–2). FIGURE 18–2 Lipase-catalyzed hydrolysis of triacylglycerol and the micellar dissolution and transport of the resulting fatty acids and 2-monoacylglycerol products.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations It is important to note that the fat must be hydrolyzed before it can be absorbed. There is no absorption through the assimilation of intact oil droplets by pinocytosis (Cardell et al., 1967). Although pancreatic lipase hydrolyzes fat only in the 1 and 3 positions of the molecule, it is nevertheless possible for fatty acids in the 2 position of the triacylglycerol to be hydrolyzed. This apparent violation of the specificity of pancreatic lipase occurs because of the relative instability of both the 2-monoacylglycerol and the 1,2-diacylglycerol (Crossley et al., 1959). These molecules rearrange by migration of the fatty acid in the 2 position to the 1 or 3 position, which is readily hydrolyzed by lipase (Figure 18–3). This rearrange- FIGURE 18–3 (A) Rearrangement of 2-monoacylglycerol and 1,2-diacylglycerol. (B) Effect of acyl migration on the hydrolysis of acylglycerols.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations rangement is more rapid when the fatty acid is either a short-chain one or an unsaturated one, and a portion of the 2-position fatty acids may be absorbed as fatty acids rather than as monoacylglycerols (Benzonana et al., 1964). In the enterocyte of the intestinal wall, high concentrations of fatty acids and monoacylglycerols are undesirable, and these compounds are rapidly converted into less toxic species, a triacylglycerol similar to that which was ingested. The 2 position of the ingested LCT is essentially maintained in the newly synthesized triacylglycerol. The fat is combined with a small amount of protein and phospholipid and is packaged in a chylomicron particle that is transported via the lymph into the blood circulation. These chylomicrons encounter lipoprotein lipase during the initial circulation, and their triacylglycerol compounds are hydrolyzed to form fatty acids that are available for utilization by the peripheral tissue. The chylomicron remnants and remaining fat are removed from the circulation by the liver for conversion into very-low-density lipoprotein. A COMPARISON OF MEDIUM- AND LONG-CHAIN FATTY ACIDS Medium-Chain Fatty Acids Fat digestion and absorption change markedly with fatty acids that contain fewer than 12 carbon atoms. There is evidence that lauric acid, with 12 carbon atoms, partly follows the absorption pattern for long-chain fatty acids and partly follows that for the 6–8- and 10-carbon fatty acids, generally classified as medium-chain fatty acids (Bragdon and Karmen, 1960) (Figure 18–4). The atypical (compared with long-chain fatty acids) digestion, absorption, and metabolism of caproic (hexanoic), caprylic (octanoic), and capric (decanoic) acids are the source of the unique properties of structured lipids. A summary of these properties lays the foundation for a review of structured lipids. Medium-chain triacylglycerols (MCTs) were first introduced to human nutrition more than three decades ago by V.K.Babayen, who was exploring ways to utilize the medium-chain fatty acids that were a by-product of the production of lauric acid from coconut oil (Senior, 1968). MCTs were prepared with these fatty acids by esterification with glycerol. Caprylic and capric acids together make up 13 percent of the fatty acids in coconut oil, and triacylglycerols made only of caprylic and capric acids account for somewhat less than 1 percent of the total oil. Thus, small amounts of MCTs have been consumed by humans for many centuries, and MCTs prepared by esterification of medium-chain fatty acids and glycerol were assumed to be generally recognized as safe. Studies of MCTs have included the early investigations of

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations digestion and absorption and more recent experiments on parenteral nutrition (Bach and Babayen, 1982). It was found that MCTs are well utilized in cases of pancreatic insufficiency that hinder the digestion and absorption of typical long-chain triacylglycerols (LCTs). Although there is some evidence that MCTs could be absorbed as intact molecules in the absence of pancreatic lipase (Clark, 1968), hydrolysis possibly takes place during the absorption of most MCTs. MCTs differ markedly from LCTs in intestinal hydrolysis in that they are hydrolyzed at a much higher rate than the LCTs (Jandacek, 1987). This rapid hydrolysis is the key event that provides the unique behavior both of MCTs and of structured lipids. MCTs are readily hydrolyzed, and their digestion products are caprylic acid, capric acid, and the 2-monoacylglycerols of these acids. One can postulate that a rapid rearrangement of the 2-monoacylglycerol to the 1-monoacylglycerol (Figure 18–3B) takes place in the small intestine so that lipase catalyzes the nearly complete hydrolysis of the MCTs. It is also possible that the 1,2-diacylglycerol produced in the stomach by gastric lipase is rearranged to the 1,3-diacylglycerol in the small intestine and is then completely hydrolyzed. The rapid hydrolysis of MCTs may in part result from the ease of removal of reaction products from the oil-water interface where the lipase is active. This prevention of rate-slowing product buildup depends on the solubilization and transport of fatty acids and monoacylglycerols by bile salt micelles in the hydrolysis of LCTs in the intestine. Although micelles may be somewhat involved in the removal of MCT digestion products, many of these products FIGURE 18–4 Description of fatty acids of various lengths. Medium-chain generally refers to fatty acids of 6, 8, or 10 carbon atoms. Long-chain fatty acids have 14 or more carbon atoms. Lauric acid (12 carbon atoms) can behave like both classes of fatty acids.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations products are removed by a fast, nonmicellar dissolution in the aqueous medium of the small intestine (Figure 18–5). The products are transported to and through the unstirred water layer coating the intestinal cell membrane without dependence on micellar dissolution. The ingestion of MCTs as a bolus does not stimulate contraction of the gallbladder, and it does not raise the plasma cholecystokinin level in the manner in which it occurs following LCT ingestion (Hopman et al., 1984; Isaacs et al., 1987). Intestinal cramping and diarrhea were observed after the MCT bolus (Hopman et al., 1984); however, the general acceptance of MCTs in mixed meals suggests amelioration by other nutrients or a development of tolerance. MCTs are also quite distinct from LCTs in the next step in the metabolic pathway. After absorption into the intestinal mucosal cell, LCT digestion products are reassembled into newly synthesized LCTs, packaged into chylomicrons, and transported via the lymph into the blood. MCT fatty acids are only sparingly found in the lymph, and there they are found only as a trace component of a mixed triacylglycerol with two long-chain fatty acids and one medium-chain fatty acid. The aqueous solubility of the MCT fatty acids causes them to enter the portal circulation bound to albumin for direct transport to the liver (Hashim, 1968) (Figure 18–6). This first encounter of MCT fatty acids FIGURE 18–5 Nonmicellar (monomeric) dissolution and transport of medium-chain fatty acids contribute to the rapid digestion and absorption of fats containing medium-chain fatty acids.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations FIGURE 18–6 Medium-chain fatty acids (MCFA) are transported in the portal vein and oxidized to form acetoacetate (ACAC) and ß-hydroxybutyrate (OHB). Long-chain fatty acids and 2-monoacylglycerol form triacylglycerols in the enterocyte and are transported as chylomicrons in the lymph. (LCT, long-chain triacylglycerol; MCT, medium-chain triacylglycerol; SL, structured lipid; OH, hydroxyl). with the liver is an important deviation from LCT metabolism, where the LCTs in chylomicrons reach the liver only after a significant portion has been hydrolyzed by lipoprotein lipase for delivery of fatty acids to peripheral tissues. It is generally accepted that the oxidation of fatty acids is facilitated by carnitine, which transports the fatty acid as an acylcarnitine into mitochondria. It has also been widely accepted that carnitine does not play a significant role in the transport of medium-chain fatty acids; however, there is now some evidence for carnitine’s involvement in the metabolism of MCTs (Rossle et al., 1990). Measurements of the levels of short-chain and long-chain acylcarnitine in the blood after infusion of a mixed MCT-LCT emulsion showed an increase in short-chain carnitine. This increase is consistent with the fact that oxidation of medium-chain fatty acids depends in part on carnitine but does not imply carnitine’s role in their transport. The difference between long-chain and medium-chain fatty acid metabolism continues in the mitochondria. There is a large body of evidence that shows medium-chain fatty acids to be preferentially oxidized in comparison with long-chain fatty acids. Scheig compared the oxidation of [1-14C] palmitic

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations acid and [1-14C]octanoic acid in rat tissues (Scheig, 1968). In the liver, kidney, diaphragm, heart, brain, and perirenal fat-pad, the extent of oxidation of octanoic acid to 14CO2 was higher than that of palmitic acid. The difference in the liver was approximately sevenfold. The epididymal fat-pad showed the same trend, but the difference was not significant. Complementary to this observation was the extent of lipid synthesis in each of these tissues. In all examined tissues except the perirenal fat-pad, more lipid was synthesized from palmitic acid than from octanoic acid, with more than a 10-fold difference seen in the liver. The oxidation of MCTs results in the production of ketones (Yeh and Zee, 1976) when fat is the principal fuel for the body, as in those receiving very-low-carbohydrate diets. The level of ketone production seen after MCT ingestion is generally far less than the high levels that are cause for concern in patients with diabetic ketosis. It has been suggested that the production of ketone bodies from MCTs is of benefit in stress situations, since they can provide ketones as an energy source for extrahepatic tissue (Birkhahn, 1988; Maiz et al., 1984). Comparison of the dietary thermogenesis resulting from meals containing LCTs and MCTs yielded results that further distinguished the metabolism of the fats on the basis of chain length. Hill and coworkers fed subjects liquid formula diets containing 150 percent of estimated energy requirements with 40 percent of calories as fat for 1 week in a double-blind crossover regimen (Hill et al., 1989). The thermal response to the MCTs was nearly twice that to the LCTs after 5 days of feeding (12±1.3 percent versus 6.6±1.0 percent) of ingested energy. The authors concluded that a principal component of this thermal effect of MCT-containing food was the energy expended in lipogenesis from the caloric excess of the MCTs. The lipogenesis from MCTs is an inefficient process with little elongation of the octanoate and, principally, the production of the acetate that is used in the de novo synthesis of fatty acids. The feeding of MCTs to animals results in virtually no deposition of octanoic and decanoic acids in the carcass or adipose tissue (Jandacek et al., 1991). Rather, palmitic acid is the dominant species. In addition to the metabolic differences between MCTs and LCTs, there are some noteworthy physical differences. The energy content (bomb calorimetry) of MCTs is approximately 8 kcal/g, in comparison with 9 kcal/g for LCTs because a higher fraction of the carbon atoms in MCTs is already bonded to oxygen and cannot be further oxidized. In the physical properties that are of importance in food preparation and consumption, MCTs are quite similar to unsaturated vegetable oils. They are, however, measurably less stable at frying temperatures and have a flash point that is markedly lower than those of typical vegetable oils (Yang, 1989). This difference results from the higher

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations volatility of the oil and the traces of lower-molecular-weight products produced during frying. Given their palatability and their relatively high caloric content when compared with those of carbohydrate and protein, MCTs have been utilized as substitutes for LCTs in the diets of patients with metabolic disorders that limit the consumption of traditional LCT fats. MCTs can be used to provide the hedonic benefit and the caloric density of fat in a meal and have been successfully used in the nutritional management of pancreatic insufficiency (Holt, 1968; Huang, 1968). However, MCTs do not provide essential fatty acids, which are particularly crucial in children. MCTs are valuable in the nutritional treatment of alcoholic pancreatitis and cholestatic liver disease; both of which decrease the concentration of bile acids below their critical micellar concentration. MCTs have also been used in patients with elevated blood triglycerides resulting from an inability to clear chylomicrons from their plasma. The MCTs can again provide the calories and palatability of fat, but they do not contribute to the formation of chylomicrons and therefore reduce the postprandial lipid levels in the blood (Furman, 1968). MCTs are currently commercially available as an over-the-counter drug in neat form. MCTs are also included in infant formula that is formulated to provide well-absorbed energy to premature infants who have poorly developed systems of fat digestive enzymes. In Europe, MCTs are sold commercially as the fat component of a diet margarine and in neat form as a salad oil for special dietary purposes. Other Dietary Fatty Acids Some recent studies with structured lipids in the diets of patients experiencing trauma-induced stress have centered not only on the mediumchain fatty acid component of the structured lipid but also on the long-chain fatty acid moiety. In particular, structured lipids with omega-3 fatty acids have been synthesized and investigated, so a review of the properties of this class of fatty acids is relevant to an understanding of structured lipids. The omega terminology for fatty acids is based on the number of carbon atoms from the terminal methyl carbon to the first double bond of the hydrocarbon chain. Omega-3 fatty acids, also called N-3 fatty acids, have a double bond three carbons from the terminal methyl group. The omega-3 fatty acids that have been the focus of hundreds of metabolic studies in the last decade are those found in marine oils, eicosapentaenoic and docosahexaenoic acids (EPA and DHA, respectively). The convenience of the omega terminology is seen in the elongation and desaturation pathways for the synthesis of

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations fatty acids in animals. The omega-3 fatty acids are precursors for other omega-3 fatty acids, and similarly, omega-6 fatty acids are precursors for elongated and desaturated omega-6 fatty acids. a-Linolenic acid is a precursor for DHA and EPA (Figure 18–7). FIGURE 18–7 The metabolism of omega-9, omega-6, and omega-3 fatty acids.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations Another potential benefit of the use of MCTs in place of carbohydrate would be the avoidance of the insulin elevation and possible hypoglycemia during exercise that follows a high-carbohydrate meal. Again, the use of medium-chain fatty acids instead of glucose for fuel could result in this effect. Although these hypotheses seem to be a reasonable mechanism for a metabolic benefit for medium-chain fatty acids resulting from MCTs or structured lipids, there has been no consistent experimental support for their validity. Table 18–2 summarizes the studies that have compared MCTs with other fuels during exercise. These studies have consistently shown a modest increase in circulating ketone bodies after ingestion of MCT-containing meals. MCT was shown in one study to blunt the increased insulin levels that occurred after ingestion of an isocaloric amount of carbohydrate, but during exercise the carbohydrate was preferentially oxidized compared with the MCTs (Howald and Decombaz, 1983). Another study did not show a reduction of insulin levels when MCTs replaced carbohydrate (Ivy et al., 1980). There was no evidence that MCTs would spare glycogen, and as a result, there was also no evidence that MCTs would extend the time to exhaustion. These studies therefore do not provide support for the use of MCTs in the enhancement of work and exercise performance. The direct extrapolation of this conclusion to structured lipids is appropriate since the only unique fuel provided by structured lipids is the medium-chain fatty acid component. Medium-chain fatty acids from structured lipids follow the same digestion and absorption process as medium-chain fatty acids from MCTs (Webb and Sanders, 1991). A clue to the inability of MCTs to spare glycogen is seen in a study in which dietary MCTs significantly enhanced dietary thermogenesis when MCTs were in excess of energy needs and compared with LCTs (Hill et al., 1989). That study raised the possibility that this thermogenesis represents energy that is uncoupled from phosphorylation so as to generate heat without work. The conversion of medium-chain fatty acids to long-chain fatty acids involves the energy-consuming process of the synthesis of fatty acids from acetate groups. OTHER CONSIDERATIONS OF MCTs AND STRUCTURED LIPIDS MCT, MCT-LCT mixes, and structured lipids may provide benefits in a nutritional regimen as part of the treatment for the stresses caused by burn injury, surgery, and infection, but there is currently little support for their use in performance enhancement. There are some other applications of the unique metabolic behaviors of precursors of medium-chain fatty acids.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations With regard to the development of chronic illness, there is evidence that MCTs do not contribute to the promotion of tumors that is seen with long-chain fatty acid triacylglycerols in experimental animals that have been challenged with a carcinogen (Cohen and Thompson, 1987). This putative benefit may result from the lower caloric density of MCT or from the minimal deposition of fatty acids in the adipose tissues of MCT-fed animals. The use of MCTs in the nutritional treatment of lipase insufficiency continues to be a viable regimen. In addition, the use of structured lipids with appropriate levels of essential fatty acids may ensure the maintenance or development of a healthful essential fatty acid status. Another interesting property of structured lipids is the ability to combine desirable physical and metabolic properties in a triacylglycerol. The formulation of a structured lipid with medium-chain fatty acids and behenic acid (22 carbons, saturated), a high-melting-point fatty acid, yields a material (caprenin) that is similar in texture and melting behavior to cocoa butter (Webb and Sanders, 1991). This hedonic benefit is coupled with hydrolysis in the intestine to produce medium-chain fatty acids and a high-melting-point species of behenic acid—the fatty acid, its soap, or the monoglyceride. The behenic compounds, because of their insolubilities and high melting points, are poorly absorbed from the intestine and are excreted. Behenate absorption from caprenin has been shown to be about 30 percent of that ingested, so the caloric density of this structured lipid is approximately 5 kcal/g, or 55 percent of the 9 kcal of typical LCTs per g. The material is therefore a unique, low-calorie substitute for cocoa butter. A technological development may produce another generation of structured lipids that have so far received little attention. Immobilized lipases have been successfully used in the synthesis of triacylglycerols with fatty acids in specific positions of the triacylglycerol structures. This process is presumably able to provide commercial quantities of specific structured triacylglycerols, and it is now possible to synthesize structured lipids with positional specificity (Jensen et al., 1987). Compounds such as the 1,3-dioctanoyl or 1,2-didecanoyl structured lipids should be available in quantity if research discloses some unique benefits attributable to these structures. CONCLUSIONS AND RECOMMENDATIONS Structured lipids are triacylglycerols synthesized from mixtures of long-chain fatty acids and medium-chain fatty acids. Structured lipids and medium-chain triacylglycerols (MCTs) have been shown to provide benefits in their applications in parenteral and enteral nutrition in the sparing of nitrogen and maintenance of reticuloendothelial system function in patients

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations TABLE 18–2 Summary of Studies of MCT Metabolism During Exercise Study Method Diet Results Decombaz and Roux (1980) Rats on treadmill Corn oil, MCT, glucose, water MCT increased ketone levels; glycogen utilization was the same in all groups; time to exhaustion was the same in all groups Ivy et al. (1980) Humans at 70 percent of maximum oxygen consumption Fasting, MCT, LCT, carbohydrate Percentage of energy from lipid was the same with MCT, LCT, and carbohydrate; MCTs did not alter the pattern of preferential oxidation of carbohydrate during exercise; serum glucose and insulin levels were similar for all groups during exercise Decombaz et al. (1983) Humans at 60 percent of maximum oxygen consumption MCT, maltodextrins (carbohydrate) Glycogen decrease was the same for MCT and carbohydrate Howald and Decombaz (1983) Humans at 60 percent of maximum oxygen consumption MCT, maltodextrins Carbohydrate was oxidized more than MCT during exercise; MCT oxidized during rest period; MCTs reduced insulin peak Sabatin et al. (1987) Humans at 60 percent of maximum oxygen consumption MCT, LCT, glucose, fasting No change in time to exhaustion; glucose was 80 percent oxidized; MCT was 45 percent oxidized, and LCT was 9 percent oxidized

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations Auclair et al. (1988) Rats on treadmill MCTs, LCTs, glucose, water MCT reduced insulin levels; ketone levels were lowest with glucose; muscle and liver glycogen levels were highest with glucose Sabatin et al. (1989) Rats on treadmill Carbohydrate, LCTs, MCTs, high protein MCT was equivalent to LCT for blood glucose, free fatty acids, and glycerol; MCT was greater than LCT for ketone body levels; MCT, LCT ketone bodies were greater than others; muscle glycogen levels were similar in all groups; liver glycogen of the MCT group was lowest Sabatin et al. (1991) Rats on treadmill Glucose, MCTs, LCTs Exercise decreased food intake in rats; food intake was decreased by LCT and MCT at 3–6 h and 0–3 h, respectively NOTE: MCT, medium-chain triacylglycerols; LCT, long-chain triacylglycerols.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations stressed by burns, surgery, and trauma. These fats can also provide energy and essential fatty acids in an absorbable form to patients with lipase insufficiency. However, there is inadequate evidence to support the use of structured lipids or MCTs to enhance exercise performance. Specific Recommendations Examinations of MCTs in exercise studies have attempted to show a reduction in glycogen depletion by prefeeding of MCTs. There have apparently not been studies of the use of MCTs or structured lipids in glycogen repletion. Although there would not seem to be an advantage over dietary carbohydrate in this regard, there are no data to confirm or refute this possibility. The studies with MCTs in exercise have generally utilized a high-exertion model. The possible sparing of glycogen by MCTs or structured lipids has not been addressed at moderate levels of work output. It is possible that the thermogenesis provided by MCTs in overfeeding could have some utility in a low-temperature environment. If this application of MCTs is beneficial, then structured lipids may provide advantages over MCTs by lessening the gastrointestinal distress resulting from the MCT bolus. Any benefits that may be discovered for MCTs in normal, healthy individuals may be better provided by structured lipids. The reduced gastrointestinal distress and the production of medium-chain fatty acids by the digestion of structured lipids suggest that structured lipids would provide the same nutrients in a more acceptable form. The chronic ingestion of structured lipids with appropriate levels of omega-3 and omega-6 essential fatty acids may provide optimum health in terms of platelet aggregation, inflammatory reactions, and the promotion of cancer. Any studies of structured lipids in this context should include comparisons with other optimum fats such as those containing high levels of oleic acid or with low-fat diets. If high-fat diets are desirable, as in the case of minimizing ration volume, then structured lipids might be advantageous compared with normal long-chain triacylglycerols (LCTs). The stomach emptying of structured lipids would be expected to be faster than that of LCTs and slower than that of MCTs. An optimum balance of caloric density and postprandial digestion might be provided.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations REFERENCES Auclair, E., P.Satabin, E.Servan, and C.Y.Guezennac 1988 Metabolic effects of glucose, medium-chain triglyceride and long-chain triglyceride feeding before prolonged exercise in rats. Eur. J. Appl. Physiol. 57:126–131. Bach, A.C., and V.K.Babayen 1982 Medium-chain Triglycerides; an update. Am. J. Clin. Nutr. 36:950–952. Benzonana, G.B., B.Entressangles, G.Marchis-Mouren, L.Pasero, L.Sarda, and P.Desnuelle 1964 Further studies on pig pancreatic lipase. Pp. 141–154 in Metabolism and Physiological Significance of Lipids, R.M.C.Dawson and D.N.Rhodes, eds. New York: Wiley. Birkhahn, R.H. 1988 Invited comment: The role of synthetic compounds in clinical nutrition. J. Parenter. Enteral Nutr. 9:559–565. Bragdon, J.H., and A.Karmen 1960 The fatty acid composition of chylomicrons of chyle and serum following the ingestion of different oils. J. Lipid Res. 1:167–170. Cardell, R.R., S.Badenhausen, and K.R.Porter 1967 Intestinal triglyceride absorption in the rat. J. Cell Biol 34:123–155. Clark, S.B. 1968 Limiting factors in maximal steady state absorption of medium chain triglycerides. Pp. 69–95 in Medium Chain Triglycerides, J.R.Senior, ed. Philadelphia: University of Pennsylvania. Clement, G., J.Clement, and J.Bezard 1962 Action of human pancreatic lipase on synthetic mixed symmetrical triglycerides of long-chain acids and butyric acid. Biochem. Biophys. Res. Commun. 8:238–242. Cohen, L.A., and D.O.Thompson 1987 The influence of dietary medium chain triglycerides on rat mammary tumor development. Lipids 22:455–461. Crossley, A., I.P.Freeman, B.J.F.Hudson, and J.H.Pierce 1959 Acyl migration in diglycerides. J. Chem. Soc. No volume:760–764. Decombaz, J., and L.Roux 1980 Glycogen utilization in exercise after increased plasma fatty acids or ketone bodies. Int. J. Vitam. Nutr. Res. 50:210–211. Decombaz, J., M.J.Arnaud, H.Milon, H.Moesch, G.Philippossian, A.L.Thelin, and H.Howard 1983 Energy metabolism of medium-chain triglycerides versus carbohydrate during exercise. Eur. J. Appl. Physiol. 52:9–14. DiMichele, S.J., M.D.Karlstad, V.K.Babayan, N.Istfan, G.L.Blackburn, and B.R.Bistrian 1988 Enhanced skeletal muscle and liver protein synthesis with structured lipid in enterally fed burned rats. Metabolism 37:787–795. DiMichele, S.J., M.D.Karlstad, B.R.Bistrian, N.Istfan, V.K.Babayan, G.L.Blackburn 1989 Enteral nutrition with structured lipid: Effect on protein metabolism in thermal injury. Am. J. Clin. Nutr. 50:1295–1302. Fernandes, J., J.H.Van de Kamer, and H.A.Weijers 1962 The absorption of fats studied in a child with chylothorax. J. Clin. Invest. 34:1026–1036. Furman, R.H. 1968 Effects of medium-chain length triglycerides on serum lipids. Pp. 51–61 in Medium Chain Triglycerides, J.R.Senior, ed. Philadelphia: University of Pennsylvania.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations Hamawy, K.J., L.L.Moldawer, M.Georgieff, and A.J.Valicente 1985 The effect of lipid emulsions on reticuloendothelial system function in the injured animal. J. Parenter. Enteral Nutr. 9:559–565. Hamosh, M., H.L.Klaeveman, R.O.Wolf, and R.O.Scow 1975 Pharyngeal lipase and digestion of dietary triglyceride in man. J. Clin. Invest. 55:908–913. Hashim, S.A. 1968 Studies of medium chain fatty acid transport in portal blood. Pp. 81–90 in Medium Chain Triglycerides, J.R.Senior, ed. Philadelphia: University of Pennsylvania. Hill, J.O., J.C.Peters, D.Yang, T.Sharp, M.Kaler, N.Abumrad, and H.L.Greene 1989 Thermogenesis in humans during overfeeding with medium-chain triglycerides. Metabolism 38:641–648. Holman, R.T. 1971 Essential fatty acid deficiency. Prog. Chem. Fats Other Lipids 9:279–348. Holt, P.R. 1968 Studies of medium chain triglycerides in patients with differing mechanisms for fat malabsorption. Pp. 97–107 in Medium Chain Triglycerides, J.R.Senior, ed. Philadelphia: University of Pennsylvania. Hopman, W.P.M., J.B.M.J.Janasen, G.Rosenbusch, and C.Lamers 1984 Effect of equimolar amounts of long-chain triglycerides and medium-chain triglycerides on plasma cholecystokinin and gallbladder contraction. Am. J. Clin. Nutr. 39:356–359. Howald, H., and J.Decombaz 1983 Nutrient intake and energy regulation in physical exercise. Experientia 44:77–88. Huang, N.N. 1968 Medium chain triglycerides in cystic fibrosis. Pp. 207–217 in Medium Chain Triglycerides, J.R.Senior, ed. Philadelphia: University of Pennsylvania. Hubbard, V.S., and M.C.McKenna 1987 Absorption of safflower oil and structured lipid preparations in patients with cystic fibrosis. Lipids 22:424–441. Innis, S.M. 1991 Essential fatty acids in growth and developmnent. Prog. Lipid Res. 30:39–103. Isaacs, P.E.T., S.Ladas, I.C.Forgacs, R.H.Dowling, S.V.Ellam, T.E.Adrian, and S.R.Bloom 1987 Comparison of effects of ingested medium- and long-chain triglycerides on gallbladder volume and release of cholecystokinin and other gut peptides. Dig. Dis. Sci. 32:481–486. Ivy, J.L., D.L.Costill, W.J.Fink, and E.Maglischo 1980 Contribution of medium and long chain triglyceride intake to energy metabolism during prolonged exercise. Int. J. Sports Med. 1:15–20. Jandacek, R.J., J.A.Whiteshide, B.N.Holcombe, R.A.Volpenhein, and J.D.Taulbee 1987 The rapid hydrolysis and efficient absorption of triglyceride with octanoic acid in the 1 and 3 position and long-chain fatty fatty acid in the 2 position. Am. J. Clin. Nutr. 45:940–945. Jandacek, R.J., J.A.Whiteside, B.N.Holcombe, C.M.Kuehlthau, J.C.Peters, and J.D. 1991 Taulbee Reduced storage of eicosapentaenoic and docosahexaenoic acids in the weanling rat. J. Nutr. Biochem. 2:142–149. Jensen, G.L., E.A.Mascioli, D.L.Seidner, N.W.Istfan, A.M.Domnitch, K.Selleck, V.K.Babayen, G.L.Blackburn, and B.R.Bistrian 1990 Parenteral infusion of long- and medium-chain triglycerides and reticuloendothelial function in man. J. Parenter. Enteral Nutr. 14:467–471.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations Jensen, R.G., J.Sampugna, R.M.Parry, and T.L.Forster 1962 Absence of fatty acid specificity during lipolysis of some synthetic triglycerides by ß-esterase preparations from milk. J. Dairy Sci. 45:842–847. Jensen, R.G., D.R.Galluzo, and B.Huge-Jensen 1987 Specificity of free and immobilized lipases from Mucor miehi. J. Am. Oil Chem. Soc. 64:644. Johnson, R.C., S.K.Young, R.Cotter, L.Lin, and W.B.Rowe 1990 Medium-chain-triglyceride lipid emulsion: Metabolism and tissue distribution. Am. J. Clin. Nutr. 52:502–508. Kasper, H. 1970 Faecal fat excretion, diarrhea, and subjective complaints with highly dosed oral fat intake. Digestion 3:321–320. Maiz, A., K.Yamazaki, J.Sobrado, V.K.Babayen, L.L.Moldawer, B.R.Bistrian, and G.L. Blackburn 1984 Protein metabolism during total parenteral nutrition (TPN) in injured rats using medium-chain triglycerides. Metabolism 33:901–915. Mattson, F.H., and R.J.Jandacek 1991 Distribution among tissues of intravenously administered sucrose octaoleate. Lipids 26:750–753. Mattson, F.H., J.C.Alexander, F.J.Baur, and H.H.Reller 1956 Short-term feeding studies on acetin fats. J. Nutr. 59:277–285. McKenna, M.C., V.S.Hubbard, and J.G.Bieri 1985 Linoleic acid absorption from lipid supplements in patients with cystic fibrosis with pancreatic insufficiency and in control subjects. J. Pediatr. Gastroenterol. Nutr. 4:45–51. Mitsuyoshi, K., Y.Hiramatsu, M.Nakagawa, M.Yamamura, K.Hioki, and M.Yamamota 1992 Effect of structured lipids as energy substrate after hepatectomy in rats with streptozocin-induced diabetes. Nutrition 8:41–46. Nakagawa, M., Y.Hiramatsu, K.Mitsuyoshi, M.Yamamura, K.Hioki, and M.Yamamato 1991 Effect of various lipid emulsions on total parenteral nutrition-induced hepatosteatosis in rats. J. Parenter. Enteral Nutr. 15:137–143. Rossle, C., Y.A.Caroentier, M.Richelle, W.Dahlan, N.P.D’attelis, P.Furst, and D.H.Elwyn 1990 Medium-chain triglycerides induce alterations in carnitine metabolism. Am. J. Physiol. 258:E944–E947. Satabin, P., P.Portero, G.Defer, J.Brecout, and C.Guezennec 1987 Metabolic and hormonal responses to lipid and carbohydrate diets during exercise in man. Med. Sci. Sports Exerc. 19:218–223. Satabin, P., B.Bois-Joyeux, M.Chanez, C.Y.Guezennec, and J.Peret 1989 Effects of long-term feeding of high-protein or high-fat diets on the response to exercise in the rat. Eur. J. Appl. Physiol. 58:583–590. Sabatin, P., E.Auclair, E.Servan, C.L.Achagiotis and C.Y.Guezennec 1991 Influence of glucose, medium- and long-chain triglyceride gastric loads and forced exercise on food intake and body weight in rats. Physiol. Behav. 50:147–150. Scheig, R. 1968 Hepatic metabolism of medium chain fatty acids. Pp. 39–49 in Medium Chain Triglycerides, J.R.Senior, ed. Philadelphia: University of Pennsylvania. Senior, J.R. 1968 Introductory remarks by the chairman. Pp. 3–7 in Medium Chain Triglycerides, J.R. Senior, ed. Philadelphia: University of Pennsylvania.

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations Sobrado, J., M.L.Moldawer, J.J.Pomposelli, E.A.Mascioli, V.K.Babayen, B.R.Bistrian, and G.L. 1985 Blackburn Lipid emulsions and reticulo-endothelial function in healthy and burned guinea pigs. Am. J. Clin. Nutr. 42:855–863. Teo, T.C., S.J.DeMichele, K.M.Selleck, V.K.Babayan, G.L.Blackburn, and B.R.Bristrian 1989 Administration of structured lipid composed of MCT and fish oil reduces net protein catabolism in enterally fed burned rats. Ann. Surg. 210:100–107. Teo, T.C., K.M.Selleck, J.M.F.Wan, J.J.Pomposelli, V.K.Babayen, G.L.Blackburn, and B.R. Bristrian 1991 Long-term feeding with structured lipid composed of medium-chain and N-3 fatty acids ameliorates endotoxic shock in guinea pigs. Metabolism 40:1152–1159. Waddell, W.R., R.P.Geyer, E.Clarke, and F.J.Stare 1954 Function of the reticuloendothelial system in removal of emulsified fat from the blood. J. Physiol. 177:747–750. Webb, D.R., and R.A.Sanders 1991 Caprenin 1. Digestion, absorption, and rearrangement in thoracic duct-cannulated rats. J. Am. Coll. Toxicol. 10:325–340. Yang, D.K. 1989 Tailored triglycerides having improved autoignition characteristics. U.S. Patent 4,832,975. Yeh, Y.Y., and P.Zee 1976 Relationship of ketosis to metabolic changes induced by acute medium-chain triglyceride feeding in rats. J. Nutr. 106:58–67. DISCUSSION GILBERT LEVEILLE: The exercise phenomenon has not been greatly studied but I would think that during aerobic exercise, when tissues would be in a relatively anaerobic state, one would predict that medium-chain triglycerides (MCTs) would be no better and, possibly, could be worse than long-chain fatty acids. And this would not prevent the depletion of glycogen. That is what people found, but apparently, no one looked at the effect of medium-chain triglycerides or structured lipids in a situation with a more moderate exercise level. RONALD JANDACEK: I have not seen that. GILBERT LEVEILLE: That would be the situation in which it should have a beneficial effect. ELDON ASKEW: On the effect of medium-chain triglycerides on thermogenesis, is this simply that the MCTs have a higher specific dynamic action? Is that what you are referring to there?

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Food Components to Enhance Performance: An Evaluation of Potential Performance-Enhancing Food Components for Operational Rations ELDON ASKEW: Nothing, though, on a substituted normal-calorie diet? RONALD JANDACEK: No. STEVEN ZEISEL: You touched in it during your talk, but could you go over the evidence that omega-3 fatty acid pretreatment seems to moderate the response to endotoxin? We know that if you are using MCT, it would be the same. RONALD JANDACEK: Not MCT. There were two studies by Teo and the Harvard group. Again, I do not think that the mechanism is very clear, other than the mechanism for those with a vasoactive effect, these omega-3 fatty acids and also an effect on the eicosanoids, that the omega-3 fatty acids do reduce the eicosanoid production that is normally seen from omega-6 fatty acids or arachidonic acid. CAROLE GREENWOOD: Question about cholesterol metabolism and structured lipids. RONALD JANDACEK: I do no think that it is. There are a couple of studies with octanoic acid. G.L.Crozier showed that octanic acid is a substrate for cholesterol synthesis. But I do not know where the break in chain length is. WILLIAM BEISEL: On the basis of what we predict from just Clyde Beany’s studies with acid, do you predict that the structured lipids will be able to deliver the omega-3 or omega-6 fatty acids to generate predictable changes in the cell wall, phagocytic cells, or lymphocytes. Has any of that been done? RONALD JANDACEK: Not with structured lipids. I think the advantage is that if you make a specific structured lipid, you can define exactly what your omega-3 fatty acid level is. There is one patent by F.Mendy in France showing some oxidative stabilization of these long-chain, highly unsaturated fatty acids by putting the medium-chain in the 1 and 3 positions. I do not know whether he proved that or not, but if that is the case, that may be an advantage because certainly the lack of hedonic benefits of fish oil fatty acids is clear.

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