that they do not result in harmful or unacceptable concentrations of toxic gases, odors, or particles in the primary enclosure. Similarly, when the calculated minimal required ventilation exceeds 15 air changes per hour, provisions should be made for additional ventilation required to address the other factors. In some cases, fixed ventilation in the secondary enclosure might necessitate adjustment of sanitation schedules or limitation of animal numbers to maintain appropriate environmental conditions.

Caging with forced ventilation that uses filtered room air and other types of special primary enclosures with independent air supplies (i.e., air not drawn from the room) can effectively address the ventilation requirements of animals without the need to ventilate secondary enclosures to the extent that would be needed if there were no independent primary-enclosure ventilation. Nevertheless, a secondary enclosure should be ventilated sufficiently to provide for the heat loads released from its primary enclosures. If the specialized enclosures contain adequate particulate and gaseous filtration to address contamination risks, recycled air may be used in the secondary enclosures.

Filtered isolation caging without forced ventilation, such as that used in some types of rodent housing, restricts ventilation. To compensate, it might be necessary to adjust husbandry practices-including sanitation, placement of cages in the secondary enclosure, and cage densities-to improve the microenvironment and heat dissipation.

The use of recycled air to ventilate animal rooms saves considerable amounts of energy but might entail some risk. Many animal pathogens can be airborne or travel on fomites, such as dust, so exhaust air to be recycled into heating, ventilation, and air-conditioning (HVAC) systems that serve multiple rooms presents a risk of cross contamination. The exhaust air to be recycled should be HEPA filtered (high-efficiency particulate air-filtered) to remove airborne particles before it is recycled; the extent and efficiency of filtration should be proportional to the estimated risk. HEPA filters are available in various efficiencies that can be used to match the magnitude of risk (ASHRAE 1992, 1993). Air that does not originate from animal use areas but has been used to ventilate other spaces (e.g., some human-occupancy areas and food, bedding, and supply storage areas) may be recycled for animal space ventilation and might require less-intensive filtration or conditioning than air recycled from animal use space. The risks in some situations, however, might be too great to consider recycling (e.g., in the case of nonhuman-primate and biohazard areas).

Toxic or odor-causing gases, such as ammonia, can be kept within acceptable limits if they are removed by the ventilation system and replaced with air that contains either a lower concentration or none of these gases. Treatment of recycled air for these substances by chemical absorption or scrubbing might be effective; however, the use of nonrecycled air is preferred for ventilation of animal use and holding areas. The use of HEPA filtered recycled air without



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