(NAS Colloquium) Molecular Kinesis in Cellular Function and Plasticity (2002) / Chapter Skim
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Pages 16-21
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From page 16... ...
The completed RNP particle is released into the nucleoplasm and transported to the nuclear pore, where the RNP fibril is gradually unfolded and passes through the pore. On the cytoplasmic side, the exiting extended RNP fibril becomes engaged in protein synthesis and the ensuing polysome is anchored to the endoplasmic reticulum.
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From page 17... ...
In fact, the proteins seem to bind to the growing RNA molecule in the immediate vicinity of the RNA polymerase. Several questions are close at hand: What proteins are associated with the RNP particle?
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From page 18... ...
3. Assemblv and transport of the BR RNP particle and its relation to a number of BR RNA-associated proteins.
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From page 19... ...
Thus, it seems likely that there is not a single protein-removal step at nucleocytoplasmic transport but rather a series of preparatory steps before the actual translocation of the RNP particle through the pore. It could be speculated that the shedding of hrp23 is required for binding of the particle to the nuclear pore complex, whereas the removal of hrp45 is closely connected to the translocation of the particle through the central channel.
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From page 20... ...
Conclusions A specific transcription product, the BR RNP particle, has been studied during assembly on the gene and transport through the nucleoplasm to and through the nuclear pores. On the cytoplasmic side, the BR RNP particle appears as an extended RNP fibril that immediately engages in protein synthesis.
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From page 21... ...
(iii) The various proteins behave differently during RNA transport: some leave the transcript in the nucleoplasm or at the nuclear pore, others are shed subsequent to the translocation of the particle through the nuclear pore, whereas still others accompany the mRNA into polysomes.
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