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Self-Perpetuating Structural States in Biology, Disease, and Genetics (2002) / Chapter Skim
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Induction and maintenance of nonsymmetrical DNA methylation in Neurospora
Pages 109-114

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From page 109...
... Methylated transforming sequences tend to remain methylated through repeated cycles of DNA replication, whereas unmethylated transforming sequences typically remain unmethylated (3-64. Strong evidence for maintenance methylation also comes from observations in Arabidopsis (7~.
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From page 110...
... Alleles shown in red were methylated afterthe cross and served as denovo methylation signals.The horizontal line atthe bottom representsthe 2.7-kb region that was sequenced, including the 2.6-kb BamHI (B) fragment.
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From page 111...
... revealed no differences indicative of methylation, indicating that wild-type am sequences are unable to maintain methylation previously established in vitro and that a high copy number of gene fragments perse does not signal DNA methylation. Size markers (kb)
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From page 112...
... Mutants of dim-2 are devoid of DNA methylation in all tissues that can be assayed, including methylation in cells that are normally capable of rapid de novo methylation (40~. To verify that DIM-2 is responsible for maintenance methylation, we first built a heterokaryon between a dim-2 deletion strain carrying an RIP-mutated allele of the mtr gene, which works as a de novo methylation signal, and a strain with a wild-type dim-2 allele carrying a deletion of the mtr gene, and confirmed de novo methylation (Fig.
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From page 113...
... 6~. The DIM-5 Histone Methyltransferase Integrates Histone Code Information and Controls DNA Methylation Results from investigating a Neurospora mutant, dim-S, which is completely defective in DNA methylation, provided the strongest evidence that histones are intimately involved in DNA methylation (42~.
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From page 114...
... Conceivably, DIM-2 could directly recruit chromatin modification factors, as in animals (51~. In any case, once methylated, the DNA should attract methyl-binding proteins, such as MRBP-1, which would again recruit chromatin modification enzymes, strengthening the epigenetic loop and resulting in the observed propagation of DNA methylation.
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Key Terms

  • maintenance methylation
  • novo methylation
  • methylated dna
  • methylation established
  • preexisting methylation

  • vegetative cells
  • histone methyltransferase
  • binding proteins
  • histone acetylation
  • symmetrical sites

  • dna methyltransferase
  • nonsymmetrical sites
  • similar factor
  • duplicated sequences
  • original transformant

  • signal dna
  • histone hypoacetylation
  • transforming sequences
  • cold spring
  • trigger dna

  • initially methylated
  • mtr gene
  • controls dna
  • methylate hemimethylated
  • chromatin modification

  • propagate methylation
  • neurospora genome
  • propagate heterogeneous
  • genome defense
  • heterogeneous methylation


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