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Self-Perpetuating Structural States in Biology, Disease, and Genetics (2002) / Chapter Skim
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Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast
Pages 62-69

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From page 62...
... Unlike in budding yeast, Set1-mediated H3 Lys-4 methylation is not required for heterochromatin assembly at the silent mating-type region and centromeres in fission yeast. Our analysis suggests that H3 Lys-4 methylation is a stable histone modification present throughout the cell cycle, including mitosis.
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From page 63...
... cerevisiae, Setl is not required for heterochromatin assembly in fission yeast, consistent with our previous results showing that H3 Lys-4 methylation is specific to euchromatic regions. H3 Lys-4-methylaton is present throughout the cell cycle and important for the upkeep of transcriptionally poised domains in euchromatic regions, perhaps through the maintenance of histone acetylation levels.
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From page 64...
... A BLAST search with the SET domain of the Clr4 histone methyltransferase revealed that at least nine SET domain proteins, including Clr4, reside in the fission yeast genome (Fig.
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From page 65...
... Deletion of set1 Does Not Affect Silencing at the Mating-Type Region and Centromeres. Modifications of histone tails are known to play a critical role in heterochromatin assembly through their role in Noma and Grewal Fig.
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From page 66...
... 45~. Previous studies have shown that marker genes inserted within or adjacent to these heterochromatic locations are subject to transcriptional repression, as a consequence of repressive chromatin complexes spreading into the marker genes (46~.
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From page 67...
... Recent studies have identified a novel class of protein methyltransferases defined by an evolutionarily well-conserved structure, the SET domain (5, 18~. In this paper, we report the structural features of at least nine SET domain proteins present in fission yeast.
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From page 68...
... Supporting the possible role for RNAs in chromatin assembly, our recent work suggests that RNA interference (RNAi) mechanisms, through which small RNAs silence cognate genes, might be required for the targeting of his/one-modifying activities to specific chromosomal domains in fission yeast.
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From page 69...
... Furthermore, methylation of H3 Lys-4 interferes with interactions between the NuRD histone deacetylase and H3 tail, as well as precludes H3 Lys-9 methylation by SUV39H1 (31, 51~. In conclusion, the results presented in this paper further extend the histone code hypothesis and suggest that Setl-mediated H3 Lys-4 methylation pr~mar~ly acts in the maintenance of active chromatin configurations at euchromatic chromosomal domains in fission yeast.
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Key Terms

  • fission yeast
  • domain proteins
  • heterochromatin assembly
  • bulk histones
  • acetylation levels

  • euchromatic regions
  • set domains
  • cell cycle
  • histone modifications
  • marker gene

  • chromatin assembly
  • active chromatin
  • structural features
  • budding yeast
  • histone tails

  • recognition motif
  • silent chromatin
  • transcriptionally poised
  • histones prepared
  • rna recognition

  • repressive chromatin
  • chromosomal domains
  • yeast cells
  • phd finger
  • pombe set

  • chromatin fractions
  • histone acetylation
  • postset domains
  • western analysis
  • amino acid


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