Self-Perpetuating Structural States in Biology, Disease, and Genetics (2002) / Chapter Skim
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Conservation of a portion of the S. cerevisiae Ure2p prion domain that interacts with the full-length protein
Conservation of a portion of the S. cerevisiae Ure2p prion domain that interacts with the full-length protein
Pages 8-15
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From page 8... ...
Sackier Colloquium of the National Academy of Sciences, "Self-Perpetuating Structural States in Biology, Disease, and Genetics," held March 22-24, 2002, at the National Academy of Sciences in Washington, DC. Abbreviations: TSE, transmissible bovine encephalopathy; GST, glutathione S-transferase; GFP, green fluorescent protein; USA, ureidosuccinate; YPAD, yeast extract peptone adenine dextrose.
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From page 9... ...
in the Netherlands (CBS400, palm wine from Elaies guineensis, Ivory Coast; CBS405, bill wine from Osbeckia grandiflora, West Africa; CBS429, fermenting must of champagne grapes; CBS2087 flower of lychee, Tonkin, China; CBS2247, grape must, Cape Province, South Africa; CBS3093, alpechin, Spain; CBS4734, from juice of sugar cane; CBS5112, grape must, Spain; CBS5287, grapes, Russia; CBS6216, Edskes and Wickner tap water, Rotterdam, The Netherlands; CBS7957, cassava flour, Sao Paulo, Brazil)
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From page 10... ...
bayanus-specific 5' oligo. Finally, the URE2 homologs were amplified by PCR using oligos that created a BamHI site followed by the nucleotides CAA upstream of the start AUG and aX7loI site immediately downstream of the stop codon.
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From page 11... ...
, suggesting that Ure2p may have been recruited into aggregates, even though these would not stably propagate. In contrast to the aggregation of overproduced Ure2p-GFP fusion proteins, their production at normal levels generally leads to aggregation only in fURE31 cells (32)
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From page 12... ...
cerevisiae Strain Changes >1278b; YJM145; YJM413; YJM280; YJM326; SAF; Fleischmann; Boots home; None McPhie sourdough; CBS2087 Lychee, China; CBS4734 Sugar cane; CBS7957 Cassava flour Brazil; German Ale YJ M320 CBS400 Palm wine-ivory Coast; CBS405 Bili wine, West Africa CBS3093 Alpechin, Spain; CBS5112 Grape must Spain CBS5287 Grapes Russia English Ale; California lager; CBS6216 tap water Roterdam A224 A264 L231 V245 N23S CBS429 grapes, France; Red Star Wine; CBS2247 Grape must, S Africa N70Y V258 YJM339 N43b S10, E260, R344 N43b means insertion of an asparagine residue after amino acid 43.
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From page 13... ...
This curing by the "hair of the dog" method has a potentially broad application, and is known to be effective in curing tissue culture cells of PrP (58~. We proposed that the Ure2p fragments or fusion proteins join the growing filaments, but do not themselves provide a growing point, thus poisoning the linear amyloid crystal, but there are other possibilities.
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From page 14... ...
It is unlikely that prion formation helps cells regulate nitrogen catabolism, because it differs from the normal regulation mainly in lacking flexibility. Although Ure2C can regulate nitrogen catabolism without the prion domain, this regulation is less efficient than that carried out by the full-length Ure2p (26, 29~.
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From page 15... ...
We thank Drs. June Kwon-Chung, John McCusker, and Peter McPhie for kindly supplying yeast strains.
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