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Regenerative Medicine (2003) / Chapter Skim
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18 Therapeutic cloning in the mouse
Pages 108-112

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From page 108...
... The culmination of the history of therapeutic cloning is a report of the first "therapeutic" effect in the final study (114: one ntES cell line was generated from 27 blastocysts generated by nuclear transfer from tail tip cells of a profoundly immunodeficient mouse, homozygous for a knockout mutation in the recombination activating gene 2. The knockout mutation was "cured" by gene targeting, and the manipulated ntES cells were differentiated in vitro into hematopoietic cells, which partially repopulated the crippled immune system of other knockout mice with the same mutation.
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From page 109...
... However, the idea of generating embryos with mixed human/ animal properties, even transiently, is offensive to many people. In the long run, efforts should be concentrated toward developing oocyte-independent systems, for instance by fusing somatic cells with enucleated ES cells, or by injecting ES cell- or oocyte-derived reprogramming factors into somatic cells.
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From page 111...
... Verma and Fred H Gage Program Friday, October 18 Keynote Speaker John Gurdon, Wellcome CRC Institute, University of Cambridge Stem Cells Saturday, October 19 Stem Cell Biology I: Species Chair, Susan Bryant Jennifer Fletcher, University of California, Berkeley Stem Cell Populations in Plants Elaine Fuchs, The Rockefeller University Beauty Is Skin Deep: Skin Stem Cells and Their Lineages*
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From page 112...
... Sunday, October 20 Regeneration I: Organogenesis Chair, Ken Chien Margaret Fuller, Stanford University Regulation of Stem Cell Turnover in Drosophila Fiona Watt, Ca ncer Resea rch U K Manipulation of the Stem Cell Compartment in Mammalian Epidermis Markus Grompe, Oregon Health Sciences University Liver Repopulation by Bone Marrow Derived Stem Cells Juan Carlos Izpisua Belmonte, The Salk Institute Regeneration and Notch Signaling Owen Witte, University of California, Los Angeles Prostate Development Regeneration Il: Tissue Engineering Chair, Shu Chien George Daley, Whitehead Institute Hematopoietic Engraftment from Embryonic Stem Cells Enables Therapeutic Cloning in a Murine Model of Immunodeficiency Ian Wilmut, Roslin Institute Cloning Jose Cibelli, Advanced Cell Technology Parthenogenesis and Therapeutic Cloning Darwin J Prockop, Tulane University School of Medicine Adult Stem Cells from Bone Marrow Stroma: Isolation, Characterization and Use in an Ex Viva Assay for Repair of Epithelium Peter Mombaerts, The Rockefeller University Cloning and Embryonic Stem Cells
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Key Terms

  • cell lines
  • stem cells
  • therapeutic cloning
  • cell line
  • nuclear transfer

  • embryonic stem
  • stem cell
  • tail tip
  • cumulus cells
  • ntes cells

  • blastocysts generated
  • somatic cells
  • differentiated cells
  • tip cells
  • reconstructed oocytes

  • lines derived
  • cell type
  • mouse ntes
  • cloned blastocysts
  • cell biology

  • knockout mutation
  • salk institute
  • progenitor cells
  • cell derivatives
  • adult stem

  • current embodiment
  • embryos develop
  • desired cell
  • gage program
  • crippled immune


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