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Regenerative Medicine (2003) / Chapter Skim
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9 Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians
Pages 45-49

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From page 45...
... , and Alejandro Sanchez Alvaradot * Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, B107 Chemical Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801; and "Department of Neurobiology and Anatomy, University of Utah School of Medicine, 401 Medical Research and Education Building, 20 North 1900 East, Salt Lake City, UT 84132 Freshwater planarian flatworms are capable of regenerating complete organisms from tiny fragments of their boLlies; the basis for this regenerative prowess is an experimentally accessible stem cell population that is present in the adult planarian.
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From page 46...
... cold cells in a planarian artificial food mixture containing homogenized liver, ultra-low gelling temperature agarose, and food coloring (Fig.
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From page 47...
... Whole-mount in situ hybridization analysis of numerous CNS-specific genes in the planarian Dugesia japonica showed that a planarian netrin gene is expressed in the brain in the presumptive visual center to which the photoreceptor axons project (23~. The planarian photoreceptors send axonal projections across the midline to the visual center of the brain; these cells can be visualized with mAb VC-1 (Fig.
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From page 48...
... 11864 1 www.pnas.org/cgi/doi/10.1073/pnas.1834205100 Discussion The above results demonstrate that feeding bacterially expressed dsRNA is an effective method for reducing gene expression in planarians. This method is specific, affecting only the gene targeted for disruption, and can be used to target genes expressed in a variety of cell types, including secretory cells, cells of the gastrovascular system, and neurons.
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From page 49...
... Kanapin, A., Le Bot, N., Moreno, S., Sohrmann, M., et al.
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Key Terms

  • optic chiasmata
  • expressed dsrna
  • cell types
  • situ hybridization
  • photoreceptor axons

  • regenerated photoreceptor
  • centrally expressed
  • bacteria expressing
  • fed bacteria
  • photoreceptor neurons

  • regenerative process
  • food coloring
  • visual center
  • axonal projections
  • stem cell

  • artificial food
  • neurons failed
  • expressing dsrna
  • ectopic projections
  • genes expressed

  • regenerative processes
  • regenerated photoreceptors
  • transcript levels
  • inhibit gene
  • positive signal

  • damon runyon
  • anterior projections
  • extend axons
  • temperature agarose
  • patterning defects


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