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Regenerative Medicine (2003) / Chapter Skim
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13 Activation of Notch signaling pathway precedes heart regeneration in zebrafish
Pages 73-79

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From page 73...
... Heart regeneration in zebrafish is accompanied by up-regulation of components of the Notch pathway, followed by members of the Msx family. These genes are not expressed during zebrafish heart development, indicating that regeneration involves the execution of a specific genetic program, rather than redeployment of a developmental program.
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From page 74...
... To evaluate the regenerative potential of zebrafish heart, we amputated the ventricular apex of adult fish and analyzed the hearts immediately after manipulation and at different time points, from 1 d to 4 mot In an initial series of experiments, we used a transgenic zebrafish line expressing the enhanced version of GFP (EGFP) under the control of the mlc2a promoter, to facilitate visualization of cells of the cardiomyocyte lineage.
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From page 75...
... Dotted lines mark the amputation plane in A-H and the estimated amputation plane in l-P, for comparison. heart regeneration, we would be able to detect molecular markers of cardiac differentiation during this process.
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From page 76...
... Together with the results from mlc2a expression and BrdUrd incorporation experiments, our findings suggest a scenario in which differentiated cardiomyocytes reenter the cell cycle and proliferate in response to heart injury, thus providing the cellular basis for epimorphic regeneration in the zebrafish heart. Up-Regulation of Msx Transcription Factors During Heart Regeneration.
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From page 77...
... Up-Regulation of Notch Pathway Components. As part of our ongoing effort to elucidate the molecular mechanisms of regeneration in zebrafish, we are performing a large-scale in situ hybridization screening of factors specifically showing altered expression during early heart regeneration and have identified a homologue of Drosophila Notch.
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From page 78...
... The data reported here provide evidence that adult zebrafish display an extraordinary capacity to regenerate extensive portions of the heart after surgical amputation. Thus, the zebrafish joins urodele amphibians as the only vertebrates described so far that can regenerate their hearts.
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From page 79...
... (1997) Tissue in Situ Hybridization: Methods in Animal Development (Wiley, New York)
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Key Terms

  • zebrafish heart
  • notch pathway
  • fin regeneration
  • cardiac development
  • transcription factors

  • adult hearts
  • situ hybridization
  • fibrin clot
  • control hearts
  • adult zebrafish

  • amputation plane
  • signaling pathway
  • msx family
  • epimorphic regeneration
  • caudal fin

  • blood clot
  • control adult
  • expression pattern
  • genetic program
  • heart amputation

  • regenerative response
  • transgenic zebrafish
  • surrounding tissue
  • embryonic development
  • limb regeneration

  • provide evidence
  • regenerating hearts
  • cell cycle
  • histological characteristics
  • molecular mechanisms


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