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Regenerative Medicine (2003) / Chapter Skim
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15 Enhanced hematopoietic differentiation of embryonic stem cells conditionally expressing Stat5
Pages 88-94

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From page 88...
... Abbreviations: ES, embryonic stem; ED, embryoicl bocly; HSC, hematopoietic stem cell; CFC, colony-forming cell; TRE, tetracycline response element; TPO, thrombopoietin; SCF, stem cell factor; VEGF, vascular enclothelial growth factor; IFS, inactivated fetal serum; MSCV, murine stem cell virus; IMDM, Iscove's modified Dulbecco's meclium. "Present aciciress: ViaCell, Inc., 26 LanciscJowne Street 580, Cambricige, MA 02139-4216.
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From page 89...
... Recombination between the chromosomal and plasmid LoxP sites, denoted by X, is mediated by transient expression of Cre recombinase. Reconstitution of neo gene function by the promoter-ATG sequence 5' to the loxP site allows for selection of successfu I i nteg ration events.
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From page 90...
... To generate an ES cell line with inducible StatS signaling, we made use of the Tet-On targeting cell line, Ainvl5 (27~. These cells constitutively express the reverse tetracycline transactivator from the ROSA26 locus, and have a tetracycline response element (TRE)
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From page 91...
... The cells were also strongly positive for CD31, a marker displayed by hematopoietic stem cells, some differentiated hematopoietic cells, as well as endothelial cells. This expression is likely hematopoietic in origin as opposed to endothelial given the compression of CD41.
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From page 92...
... 1734140100 makes ES cells promising source material for regenerative medicine. However, putting this potential into practice in adults, as opposed to embryos, will require the derivation of adultrepopulating stem cells from ES cells in vitro.
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From page 93...
... By analysis of surface marker expression, the StatSexpanded cells described here are closer in type to those expanded by HoxB4, in particular in their expression of CD31, CD41, and c-Kit, and in the presence of differentiating cells expressing myeloid lineage markers. The Bcr/Abl-expanded cells were negative for all lineage markers tested, although we did observe lineage marker expression on some cells in the earliest phase of expansion.
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From page 94...
... An attractive possibility is that Stats modulates the rate of progression from stem cell to committed progenitor (CFC) to differentiated progeny within the early embryonic hematopoietic compartment such that stem cells and progenitors accumulate to greater numbers in the presence of .
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Key Terms

  • cell line
  • stem cell
  • stats activation
  • embryonic stem
  • stem cells

  • embryonic hematopoietic
  • hematopoietic progenitors
  • hematopoietic lineage
  • hematopoietic stem
  • hematopoietic differentiation

  • response element
  • hematopoietic development
  • blast cells
  • cell population
  • constitutively active

  • tetracycline response
  • definitive hsc
  • active mutant
  • loxp site
  • blast cell

  • lineage markers
  • hematopoietic tissue
  • definitive hscs
  • fetal serum
  • mouse embryonic

  • yolk sac
  • results demonstrate
  • growth factor
  • extracellular signals
  • plated onto


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