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Regenerative Medicine (2003) / Chapter Skim
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16 Nonhuman primate parthenogenetic stem cells
Pages 95-100

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From page 95...
... Sackler Colloquium of the National Academy of Sciences, "Regenerative Medicine," held October 18-22, 2002, at the Arnold and Mabel Beckman Center of the National Academies of Science and Engineering in Irvine, CA. Abbreviations: ES cell, embryonic stem cell; Cyno-1 cell, parthenogenetically derived stem cell line from the cynomolgus macaque; bFGF, basic fibroblast growth factor; Snrpn, small nuclear ribonucleoprotein polypeptide N; TUJ1, ,8-tubulin lil; PBL, peripheral blood Iymphocytes; ICM, inner cell mass.
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From page 96...
... were cultured on a feeder layer of mitotically inactive mouse embryonic fibroblasts in Dulbecco's minimal essential medium with 15% FCS (HyClone)
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From page 97...
... In vitro differentiation was induced by isolating the cells from the mouse feeder layer and culturing them in the presence of Dulbecco's minimal essential medium with 15% FCS; in some instances, 1,000 units of leukemia inhibitory factor was added to the media. A large variety of specialized cell types could be generated in vitro, such as spontaneously beating cardiomyocyte-like cells and ciliated epithelium, smooth muscle cells and cytokeratin-positive cells, as well as neuronal cells (data not shown)
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From page 98...
... One of many mitotic figures. Immunohistochemical analyses after 5 days of neural differentiation in the absence of bFGF and epidermal growth factor and the presence of ascorbic acid revealed positive staining for glial fibrillary acidic protein (GFAP)
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From page 99...
... . Micro SSPTM Generic HLA Class II DNA typing was performed in a 96-well tray format through the Wake Forest University-Baptist Medical Center Histocompatibility Laboratory.
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From page 100...
... First, ES cells are isolated from the blastocyst stage, this stage exhibits low DNA methylation levels, and the effects of imprinting could be minimized (34~. Second, imprinting, in some cases, has been shown to not be completely silent: there are reports of mRNA expressed from imprinted genes that should not have been transcribed (244.
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Key Terms

  • telomerase activity
  • tumor rejection
  • rejection antigen
  • neural cells
  • growth factor

  • parthenogenetic stem
  • feeder layer
  • mouse feeder
  • stem cell
  • neural precursors

  • nonhuman primate
  • cell line
  • neural precursor
  • ascorbic acid
  • imprinted genes

  • parthenogenetic origin
  • embryonic stem
  • combined immunodeficient
  • cell types
  • imprinted gene

  • severe combined
  • parthenogenetically derived
  • primate parthenogenetic
  • minimal essential
  • immunodeficient mice

  • epidermal growth
  • activated eggs
  • flow cytometry
  • essential medium
  • cells express


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