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2 Test Protocol
Pages 11-29

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From page 11...
... The currently accepted CB protective suit, referred to as the battle ctress overgarment, is constructed from a permeable, multilayerect material that incorporates activated charcoal to absorb chemical agents. A permeable outer layer of fabric (a tri-blenct of 58 percent cotton, 27 percent Keviar aramict, anct 15 percent nylon twill weave)
From page 12...
... A battery of laboratory tests have been clevelopect anct are available to evaluate the performance of cancticiate materials. These tests inclucle liquid anct vapor chemical agent permeation tests through fabric samples mounted on diffusion cells.
From page 13...
... In recognition of this fact, the MIST program has been clevelopect to evaluate inctiviclual CB protective ensembles uncler realistic, dynamic conditions. The essential elements of the MIST involve placing inctivicluals wearing cancticiate suits in an enclosect environment Referred to as a defensive test chamber)
From page 14...
... Biological monitoring is now being wiclely used to assess worker exposure to pesticides anct other hazardous chemicals found in the workplace anct has become a benchmark for stanciarctizing other procedures for assessing worker exposure (Wang et al., 19891. For obvious reasons, biological monitoring cannot be used directly in human studies to evaluate protective materials against CB agents.
From page 15...
... . explre(1 air Ease of sampling Simple analysis Quantitative assessment of skin deposition Integrates body exposure, skin deposition absorption, and possibly inhalation; Closest estimate of total body dose Extrapolation error Provides no skin absorption data Provides no skin absorption data Requires information on distribution or metabolism The biggest advantage of the air sampling technique is the simplicity of direct chemical analysis.
From page 16...
... In contrast, biological monitoring determines an absorbect close anct would be most useful for assessing a soictier's effectiveness. MIST Test Procedure The MIST anct BRHA were clevelopect to provide a system-level evaluation of CB protective suits (Feclele anct Nelson, 19961.
From page 17...
... Boot (left) The test chamber is a stainless steel, environmentally-controllect unit that can accommodate eight test subjects.
From page 18...
... . ~ FIGURE 2-2 MIST system test chamber.
From page 19...
... When exposure to VX vapor is being simulatect, the BRHA is "systemic" because VX toxicity results from cumulative absorption through exposed skin. The mass of VX required to produce systemic toxicity after exposure to various regions of the skin was clerivect from the work of Sim (1962)
From page 20...
... For exposure to HO vapor, the At factor necessary to produce equivalent toxicity at various anatomic locations is based on a vapor exposure of 1,000 mg/m3-min to a human forearm causing severe burns in hot (above 27°C) , humid conditions.
From page 21...
... is a standard military uniform not specifically designed for chemical protection. Table 2-5 contains the I°ge transform of the geometric mean and standard deviation contained in Table 2-4, as well as the 95 percent confidence intervals (95 percent CIs)
From page 22...
... 2,845 93.82,346 Knees (front) 200 1022,550 Anatomical site, surface area of anatomical site, dose of VX that would cause a 70 decent depression in red blood cell cholinesterase, and estimated local exposure to HD (mg/m -min)
From page 23...
... 6 1,930 2.08 26 Duty uniform, non-fire resistant, model 1 (w) 6 1,823 3.82 28 Standard Army chemical protective undergarment (w)
From page 24...
... -- -- ) 4.0 6.0 8.0 10.0 aNumber of test replicates for each ensemble bPooled standard deviation = 1.0
From page 25...
... Table 2-8 shows the total mass of methyl salicylate collectect in the passive samplers anct the geometric mean of the local effective At (local analysis for HD) for the 41 protective ensembles (see also Figure 2-31.
From page 26...
... r~:~:-:-:-~:^n-_r.-~:-:-~.~41:~t~-~:~:-:-:-~-1~-l-:-:-:~-:-:(-:-n-~:-~:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-~-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-:-: ~ ~ ~ ~ ~ ,, ~ ~ ...................... 40 Saratoga chemical protective suit (worn over personal undergarments only or over duty uniform (nr)
From page 27...
... -- -- ) 4.5 6.0 7.5 aNumber of test replicates for each ensemble bPooled standard deviation = 0.6
From page 28...
... Collected in Samplers Geometric Mean of Local Effective ct(mg/m3-min) Duty, non-fire resistant, model 3 25.1 5,690 Duty uniform, non-fire resistant, model 2 28.7 5,320 Vapor protective, fire resistant, protective undergarment 32.4 2,490 Saratoga chemical protective suit (worn over personal undergarments only or over duty uniform (w)
From page 29...
... FIGURE 2-3 Local effect At versus total sampler mass.


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