Assessment of Future Scientific Needs for Live Variola Virus (1999) / Chapter Skim
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8 Detection and Diagnosis
8 Detection and Diagnosis
Pages 59-62
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From page 59... ...
Such approaches would be dependent on knowledge of the range of variability in natural variola sequences and/or the sequences of their encoded proteins. It is likely that from current knowledge of the sequences of individual orthopoxvirus genes and from the complete sequences of three variola major virus isolates that are available, polymerase chain reaction (PCR)
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From page 60... ...
Little is known about the range of DNA sequence variability among isolates of monkeypox virus. Concerns analogous to those regarding DNA sequence conservation and variability would hold for protein- or antibody-based detection or diagnostic strategies.
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From page 61... ...
However, initial epidemiological or medical interventions might be indicated solely upon finding orthopoxvirus-specif~c nucleic acid in human material in a setting in which variola virus infection was a possibility. Although technology for detecting antibodies to variola virus would be of limited or no utility in the first few days after exposure, robust antibody detection schemes might be highly useful for epidemiological surveillance following an outbreak.
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From page 62... ...
A vaccinia virus recombinant containing all or part of the relevant variola virus DNA segment would be useful for the validation of tests in appropriate epidemiological or experimental animal infection model systems, although assessment of the authenticity of the recombinant as compared with live variola virus would be needed. Parallel work with monkeypox virus might add a small margin of additional validation.
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