Assessment of Future Scientific Needs for Live Variola Virus (1999) / Chapter Skim
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9 Bioinformatics
9 Bioinformatics
Pages 63-68
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From page 63... ...
For example, a recent outbreak of monkeypox exhibited somewhat greater humanto-human transmission than had been the case in the past. However, preliminary results from sequencing of DNA fragments of monkeypox virus isolates obtained at various times since 1970 suggest that the virus has changed very little over this period.
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From page 64... ...
While only 18 of 200 proteins in the entire genome differ significantly between the Bangladesh and India strains, 7 of 30 open reading frames at the left terminus and 8 of 22 open reading frames near the right terminus show variation between the two viruses [36~. It must be remembered that a very minor change a single base addition or deletion or a single amino acid coded by a gene can lead to profound effects in the corresponding proteins that determine variations in virulence.
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From page 65... ...
The analysis showed that the most varied proteins arise from the DNA terminal regions. Interestingly, the group of ORF A14LA32L encodes proteins with greater amino acid diversity than does the group A33LA49R, which is more proximal to the right end of the BSH and IND Hind III map.
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From page 66... ...
This capability would have profound implications for issues of security, safety, and ethics surrounding the proposed destruction of live variola virus stocks. The ability to reconstitute viruses from DNA clones would make it possible to engineer a variety of attenuated viruses that would constitute an effective form of biological containment to help ensure safety while working with live virus.
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From page 67... ...
BIOINFORMA TICS 67 from the published sequence information. There is no way of predicting the rate at which such technologies might develop.
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