Assessment of Future Scientific Needs for Live Variola Virus (1999) / Chapter Skim
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Executive Summary
Executive Summary
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Research using variola therefore offers the potential to contribute to mankind's knowledge of the human body's uniquely evolved system of defense against infection. SCOPE In preparation for international deliberations concerning whether to destroy all known variola virus stocks, stored clinical materials containing live variola virus, and variola virus intact genome DNA held in the two international repositories, this committee was asked to assess future scientific needs for live variola virus.
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Although some insight into variation might be obtained by restriction fragment length polymorphism comparison of variola genes amplified by polymerase chain reaction, the precise nature of individual gene variation and resultant impact on the protein products requires more detailed sequencing. Given that the current policy is to destroy the stocks of vario}a virus in 1999, this need is urgent.
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Vaccines derived from tissue culture could be compared with the standard vaccine by evaluation in human subjects and by laboratory assays. Live variola virus would be required only for testing of novel vaccine development strategies using materials other than live vaccinia virus.
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The specific spatial and temporal patterns of variola virus gene expression must be deciphered in the context of infection at the level of cells, organs, and animal models. Studies of these phenomena could provide information on how the virus manipulates the human immune response in order to spread, on the mechanisms of cell death, and on other aspects of variola infection.
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