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Monoclonal Antibody Production (1999) / Chapter Skim
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In Vitro Production of Monoclonal Antibody
Pages 12-15

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From page 12...
... Many commercially available devices have been developed for in vitro cultivation. These devices vary in the facilities required for their operation, the amount of operator training required, the complexity of operating procedures, final concentration of antibody achieved, cost, and fluid volume accommodated.
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From page 13...
... Methods that increase the concentration of dissolved oxygen in the medium may increase cell viability and the density at which the cells grow and thus increase mAb concentration (Boraston and others 1984; Miller and others 1987~. Some of those methods use spinner flasks and roller bottles that keep the culture medium in constant circulation and thus permit nutrients and gases to distribute more evenly in large volumes of cell-culture medium (Reuveny and others 1986; Tarleton and Beyer 1991~.
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From page 14...
... The advantage of membrane-based systems is that high concentrations of mAb can be produced in relatively low volumes and fetal calf serum can be present in the media reservoir with only insignificant crossover of bovine immunoglobulins into the cell chamber. A disadvantage is that the mAb may be contaminated with dead cell products.
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From page 15...
... If investigators are unable to invest the time or material costs, several institutional core facilities and government and commercial contract laboratories produce mAb from a hybridoma. For example, commercial contract laboratories typically charge $11/mg to produce 1,000 mg with hollow-fiber reactors (Chandler, 1998~.
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Key Terms

  • culture medium
  • mouse ascites
  • mab concentration
  • ascites method
  • bovine serum

  • bovine immunoglobulin
  • increase mab
  • monoclonal antibody
  • commercial contract
  • contract laboratories

  • production methods
  • producing mab
  • hybridoma cell
  • mab concentrations
  • scientific justification

  • fetal bovine
  • hybridoma cells
  • unpurified antibodies
  • typical membrane
  • vitro methods

  • institutional core
  • contains hybridoma
  • cutoff filtration
  • increase cell
  • low volumes

  • numerous situations
  • simplest approach
  • volume accommodated
  • hybridoma culture
  • low startup


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