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Monoclonal Antibody Production (1999) / Chapter Skim
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Scientific Needs for Mouse Ascites Production of mAb
Pages 16-21

The Chapter Skim interface presents what we've algorithmically identified as the most significant single chunk of text within every page in the chapter.
Select key terms on the right to highlight them within pages of the chapter.


From page 16...
... That is consistent with the 3% failure rate observed by Dutch scientists (Hendriksen and others 1996~. A recent European workshop discussed the effects of restrictions on the ascites method in various European countries; each country's laws provide for an exception based on the inability of a hybridoma to grow and produce mAb in vitro.
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From page 17...
... Transfectomas myeloma lines transfected with mutated antibody sequences, which are often used to determine structure-function relationships are notoriously low antibody producers. In general, the only way to obtain adequate amounts of antibody for experimental study from such lines is to use the ascites method.
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From page 18...
... Although some rat hybridomas adapt to in vitro conditions, this often requires tedious manipulation of the culture. When small volumes of concentrated rat mAb are needed and the hybridoma does not easily adapt to culture conditions, the mouse ascites method using immunocompromised mice is required (Wolf 1998~.
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From page 19...
... Adapting hybridoma cell lines, initially approved for ascites-generated mAb, to serum-free conditions requires the hybridoma owner to demonstrate analytic comparability. Alterations in mAb binding affinity or other biologic functions could result in expenditure of millions of dollars (Maxim 1998~.
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From page 20...
... Although Roggenbuck and others (1994) produced milligram quantities of polyreactive IgM mAb with in vitro methods, 1% FBS in the media was required, and reactions between the IgM mAb and other components of the media led to impaired solubility of the antibody and poor reproducibility of purification results.
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From page 21...
... Yeast, fungal, or mycoplasma contamination of in vitro cultures of hybridoma can be removed by passing cells from the culture through mice. Removal of the organisms cannot be accomplished by current antimicrobial drugs.
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Key Terms

  • mouse ascites
  • cell lines
  • vitro methods
  • mab production
  • culture conditions

  • vitro conditions
  • hybridoma cell
  • infectious agents
  • human diseases
  • produce mab

  • generate ascites
  • igg mab
  • vitro production
  • igm mab
  • rat hybridoma

  • vitro culture
  • producing mab
  • immunocompromised mice
  • hybridoma cells
  • media led

  • contained terminal
  • downstream purification
  • vitro glycosylation
  • standard procedures
  • mouse proteins

  • vitro growth
  • obtain adequate
  • notoriously low
  • binding affinities
  • tumor antigens


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