Monoclonal Antibody Production (1999) / Chapter Skim
Currently Skimming:
Summary of Advantages and Disadvantages of In Vitro and In Vivo Methods
Summary of Advantages and Disadvantages of In Vitro and In Vivo Methods
Pages 22-24
The Chapter Skim interface presents what we've algorithmically identified as the most significant single chunk of text within every page in the chapter.
Select key terms on the right to highlight them within pages of the chapter.
From page 22... ...
As noted below under the section titled "In Vivo and In Vitro Methods for Commercial Production of mAb", in vitro methods can cost l/2 to 6 times the mouse ascites method. Some of the factors that cause in vitro production to be expensive are labor and equipment costs that are usually due to poor hybridoma production of mAb in vitro.
|
From page 23... ...
Note that semipermeable-membrane-based systems have been developed that can produce concentrations of mAb comparable with concentrations observed in mouse ascites fluid. · In batch tissue-culture methods, mAb concentration tends to be low in the supernatant; this necessitates concentrating steps that can change antibody affinity, denature the antibody, and add time and expense.
|
From page 24... ...
· MAb produced by in vivo methods can contain various mouse proteins and other contaminants that might require purification. · The mouse ascites method can be expensive if immunodeficient mice in a barrier facility must be used.
|
Key Terms
This material may be derived from roughly machine-read images, and so is provided only to facilitate research.
More
information on Chapter Skim is available.