Microbial Status and Genetic Evaluation of Mice and Rats Proceedings of the 1999 US/Japan Conference (2000) / Chapter Skim
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Evaluation of Targeted Mutations
Evaluation of Targeted Mutations
Pages 116-118
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From page 116... ...
The approach has also been the outcome of a deliberate increase in the working relationship between our discovery group; our core genetic facility that produces transgenic, knock-in, and knock-out mice; our pathologists; and our laboratory animal clinicians, who are responsible for the care of these mice. A typical core team consists of a principal investigator (a biologist from any area in drug discovery)
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From page 117... ...
Our methods of choice are, as usual, Northern blots or reverse transcription polymerase chain reaction. For our primary characterization of changes in gene expression, we use Western blots and enzyme-linked immunosorbent assay primarily for analyzing the changes in protein expression.
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From page 118... ...
We run a routine clinical chemistry panel, hematology, and other special tests as needed, such as hormones or cytokines, again depending on the target gene. The best scientific practices for accurate phenotyping require experienced molecular laboratories.
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