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Microbial Status and Genetic Evaluation of Mice and Rats Proceedings of the 1999 US/Japan Conference (2000) / Chapter Skim
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Factors Causing Difficulties in Uniformity of Results Among Testing Facilities in Microbiologic Monitoring of Laboratory Animals
Pages 16-20

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From page 16...
... In culture tests, discrepancies in results have often been observed. To test for this organism, we coat swabs from the trachea and conjunctive on horse blood agar plates and ultimately identify suspected colonies by Gram staining, testing of characteristics by the API system, and DNA testing by polymerase chain reaction (PCR)
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From page 17...
... When we tested some of the animals that were positive in the first test, we obtained consistently negative results. It is evident that results of bacteriologic tests using cultures will show discrepancies if there are differences in sampling sites, test methods, and identification criteria.
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From page 18...
... With the usual serologic test methods for Tyzzer's disease, ELISA and IFA, there appear to be no marked differences in use among testing facilities. However, a detailed investigation revealed differences in strains, antigen preparation, and evaluation of results.
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From page 19...
... Antibody testing of this virus was possible in several facilities in Japan at the time of the outbreak. The ICLAS Monitoring Center sent technicians to Fort Detrick in the United States with an introduction from Dr.
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From page 20...
... The position taken by the testing facility concerning its level of responsibility when submitting test results will also influence the results. I am well aware that test methods are in a constant state of development, test facilities take pride in their techniques, and it is very difficult to achieve uniform test methods.
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Key Terms

  • laboratory animals
  • antibody testing
  • monitoring center
  • iclas monitoring
  • testing facilities

  • antibody tests
  • antibody titer
  • microbiologic monitoring
  • test items
  • animal facilities

  • sera reacted
  • confirmation test
  • laboratory animal
  • nonspecific reactions
  • low antibody

  • accurate identification
  • antibody titers
  • using ifa
  • indirect immunofluorescent
  • method based

  • serum samples
  • performed using
  • blood agar
  • constant state
  • gram staining

  • experimental animals
  • test followed
  • antibody test
  • neutralization test
  • virus follows


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